Single-wavelength functional photoacoustic microscopy in biological tissue

Amos Danielli, Christopher P. Favazza, Konstantin Maslov, Lihong V. Wang

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

Recently, we developed a reflection-mode relaxation photoacoustic microscope, based on saturation intensity, to measure picosecond relaxation times using a nanosecond laser. Here, using the different relaxation times of oxygenated and deoxygenated hemoglobin molecules, both possessing extremely low fluorescence quantum yields, the oxygen saturation was quantified in vivo with single-wavelength photoacoustic microscopy. All previous functional photoacoustic microscopy measurements required imaging with multiple-laser- wavelength measurements to quantify oxygen saturation. Eliminating the need for multiwavelength measurements removes the influence of spectral properties on oxygenation calculations and improves the portability and cost-effectiveness of functional or molecular photoacoustic microscopy.

Original languageEnglish
Pages (from-to)769-771
Number of pages3
JournalOptics Letters
Volume36
Issue number5
DOIs
StatePublished - 1 Mar 2011
Externally publishedYes

Fingerprint

Dive into the research topics of 'Single-wavelength functional photoacoustic microscopy in biological tissue'. Together they form a unique fingerprint.

Cite this