Single-step conjugation of antibodies to quantum dots for labeling cell surface receptors in mammalian cells

Gopal Iyer; Jianmin Xu; Shimon Weiss

doi:10.1007/978-1-61779-151-2_34

Single-step conjugation of antibodies to quantum dots for labeling cell surface receptors in mammalian cells

Gopal Iyer, Jianmin Xu, Shimon Weiss

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Scopus citations

Abstract

Labeling of cell surface receptors in living cells can be achieved using antibody-conjugated semiconductor quantum dots (QDs). The inherent photostable property of QDs can be exploited for understanding the arrangement and distribution of receptors in the plasma membrane. We describe herein methods that allow conjugation of antibodies to QDs in a single step without the formation of side products. This protocol can be adapted universally for any type of QD structure with a coating of free amino groups.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	553-563
Number of pages	11
DOIs	https://doi.org/10.1007/978-1-61779-151-2_34
State	Published - 2011
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	751
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC 2011.

Funding

These protocols were developed in part by support from grants NIH/NIBIB BRP5-R01-EB000312 and NIH1-R01-GM086197-01. Ensemble confocal imaging was performed at the UCLA/CNSI Advanced Light Microscopy Shared Facility.

Funders	Funder number
NIH/NIBIB	NIH1-R01-GM086197-01, BRP5-R01-EB000312
UCLA/CNSI Advanced Light Microscopy Shared Facility
National Institute of General Medical Sciences	R01GM086197
National Institute of Biomedical Imaging and Bioengineering	R01EB000312

Keywords

Antibodies
Cell membrane labeling
Epidermal growth factor receptor
Gel filtration
Live cell imaging
Peptides
Quantum dot

Access to Document

10.1007/978-1-61779-151-2_34

Cite this

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Single-step conjugation of antibodies to quantum dots for labeling cell surface receptors in mammalian cells

Abstract

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Bibliographical note

Funding

Keywords

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