Labeling of cell surface receptors in living cells can be achieved using antibody-conjugated semiconductor quantum dots (QDs). The inherent photostable property of QDs can be exploited for understanding the arrangement and distribution of receptors in the plasma membrane. We describe herein methods that allow conjugation of antibodies to QDs in a single step without the formation of side products. This protocol can be adapted universally for any type of QD structure with a coating of free amino groups.
|Title of host publication||Methods in Molecular Biology|
|Publisher||Humana Press Inc.|
|Number of pages||11|
|State||Published - 2011|
|Name||Methods in Molecular Biology|
Bibliographical noteFunding Information:
These protocols were developed in part by support from grants NIH/NIBIB BRP5-R01-EB000312 and NIH1-R01-GM086197-01. Ensemble confocal imaging was performed at the UCLA/CNSI Advanced Light Microscopy Shared Facility.
© Springer Science+Business Media, LLC 2011.
- Cell membrane labeling
- Epidermal growth factor receptor
- Gel filtration
- Live cell imaging
- Quantum dot