TY - JOUR
T1 - Single-Pixel Photoacoustic Microscopy with Speckle Illumination
AU - Caravaca-Aguirre, Antonio M.
AU - Poisson, Florian
AU - Bouchet, Dorian
AU - Stasio, Nicolino
AU - Moreau, Philippe
AU - Wang, Irene
AU - Zhang, Edward
AU - Beard, Paul
AU - Prada, Claire
AU - Moser, Christophe
AU - Psaltis, Demetri
AU - Katz, Ori
AU - Bossy, Emmanuel
N1 - Publisher Copyright:
© 2023 Antonio M. Caravaca-Aguirre et al.
PY - 2023
Y1 - 2023
N2 - Wide-field optical-resolution microscopy with structured illumination and single-pixel detection has been the topic of a number of research investigations. Its advantages over point scanning approaches are many and include a faster acquisition rate for sparse samples, sectioning, and super-resolution features. Initially introduced for fluorescence imaging, structured illumination approaches have been adapted and developed for many other imaging modalities. In this paper, we illustrate how speckle illumination, as a particular type of structured illumination, can be exploited to perform optical-resolution photoacoustic microscopy with a single-pixel imaging approach. We first introduce the principle of single-pixel detection applied to photoacoustic imaging and then illustrate in 2 different situations how photoacoustic images may be computationally reconstructed from speckle illumination: In the first situation where the speckle patterns are known through a prior calibration, various reconstruction approaches may be implemented, which are demonstrated experimentally through both scattering layers and multimode optical fibers; in the second situation where the speckle patterns are unknown (blind structured illumination), the so-called memory effect can be harnessed to produce calibration-free photoacoustic images, following the approach initially proposed for fluorescence imaging through thin scattering layers.
AB - Wide-field optical-resolution microscopy with structured illumination and single-pixel detection has been the topic of a number of research investigations. Its advantages over point scanning approaches are many and include a faster acquisition rate for sparse samples, sectioning, and super-resolution features. Initially introduced for fluorescence imaging, structured illumination approaches have been adapted and developed for many other imaging modalities. In this paper, we illustrate how speckle illumination, as a particular type of structured illumination, can be exploited to perform optical-resolution photoacoustic microscopy with a single-pixel imaging approach. We first introduce the principle of single-pixel detection applied to photoacoustic imaging and then illustrate in 2 different situations how photoacoustic images may be computationally reconstructed from speckle illumination: In the first situation where the speckle patterns are known through a prior calibration, various reconstruction approaches may be implemented, which are demonstrated experimentally through both scattering layers and multimode optical fibers; in the second situation where the speckle patterns are unknown (blind structured illumination), the so-called memory effect can be harnessed to produce calibration-free photoacoustic images, following the approach initially proposed for fluorescence imaging through thin scattering layers.
UR - http://www.scopus.com/inward/record.url?scp=85192919157&partnerID=8YFLogxK
U2 - 10.34133/icomputing.0011
DO - 10.34133/icomputing.0011
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AN - SCOPUS:85192919157
SN - 2771-5892
VL - 2
JO - Intelligent Computing
JF - Intelligent Computing
M1 - 0011
ER -