TY - CHAP
T1 - Single-particle tracking for studying the dynamic properties of genomic regions in live cells
AU - Berger, Irena Bronshtein
AU - Kepten, Eldad
AU - Garini, Yuval
PY - 2013
Y1 - 2013
N2 - The appropriate functioning of living cells depends on a variety of dynamic processes that necessitate delicate motion, transportation, association, and disassociation in time and space. Different dynamic patterns such as directed motion, normal diffusion, and restricted diffusion take part at different length scales, and their identification serves as a tool for exploring biochemical processes. Here we describe single-particle tracking which is a powerful method that allows the characterization of dynamic processes on the single-molecule or single-particle level with nanometer spatial and sub-second temporal precision. In particular, we describe the cell preparation procedures, microscopy imaging, and image analysis processes for following telomere dynamics in living mammalian cells.
AB - The appropriate functioning of living cells depends on a variety of dynamic processes that necessitate delicate motion, transportation, association, and disassociation in time and space. Different dynamic patterns such as directed motion, normal diffusion, and restricted diffusion take part at different length scales, and their identification serves as a tool for exploring biochemical processes. Here we describe single-particle tracking which is a powerful method that allows the characterization of dynamic processes on the single-molecule or single-particle level with nanometer spatial and sub-second temporal precision. In particular, we describe the cell preparation procedures, microscopy imaging, and image analysis processes for following telomere dynamics in living mammalian cells.
KW - Chromatin
KW - Diffusion
KW - Fluorescence microscopy
KW - Nuclear organization
KW - Single-particle tracing
UR - http://www.scopus.com/inward/record.url?scp=84934440612&partnerID=8YFLogxK
U2 - 10.1007/978-1-62703-526-2_10
DO - 10.1007/978-1-62703-526-2_10
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C2 - 23980005
AN - SCOPUS:84934440612
SN - 9781627035255
T3 - Methods in Molecular Biology
SP - 139
EP - 151
BT - Imaging Gene Expression
PB - Humana Press Inc.
ER -