Single-Pair Courtship and Competition Assays in Drosophila

Anne C. von Philipsborn; Galit Shohat-Ophir; Carolina Rezaval

doi:10.1101/PDB.PROT108105

Single-Pair Courtship and Competition Assays in Drosophila

Anne C. von Philipsborn, Galit Shohat-Ophir, Carolina Rezaval

The Mina and Everard Goodman Faculty of Life Sciences

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Courtship in Drosophila melanogaster involves a series of innate, complex behaviors that allow male and female flies to exchange sensory information and assess the quality of a potential mate. Although highly robust and stereotypical, courtship behaviors can be modulated by internal state and experience. This protocol describes methods for designing and carrying out experiments that measure courtship performance in single-pair assays, in which a male is paired with a female, or in competitive assays, in which a male is presented with a female and with a male competitor. It also includes approaches for measuring female sexual receptivity during courtship.

Original language	English
Pages (from-to)	450-459
Number of pages	10
Journal	Cold Spring Harbor Protocols
Volume	2023
Issue number	7
DOIs	https://doi.org/10.1101/PDB.PROT108105
State	Published - 5 Jul 2023

Bibliographical note

Publisher Copyright:
© 2023 Cold Spring Harbor Laboratory Press.

Funding

We thank Laurie Cazale-Debat, Shaleen Glasgow, and Saloni Rose for providing schematics. We are grateful to Dana Galili, Assa Bentzur, and Julia Ryvkin for insightful comments on this text. Work in our laboratory was supported by the Lundbeckfonden under grant number DANDRITE R248-2016-2518 to A.C.v.P., the Israel Science Foundation Grant 174/19 to G.S.-O., and BBSRC (BB/S009299/1), Wellcome Trust (214062/Z/18/Z), and Royal Society Research (RGS\R2\180272) grants to C.R.

Funders	Funder number
Royal Society Research	RGS\R2\180272
Wellcome Trust	214062/Z/18/Z
Biotechnology and Biological Sciences Research Council	BB/S009299/1
Lundbeck Foundation	DANDRITE R248-2016-2518
Israel Science Foundation	174/19

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10.1101/PDB.PROT108105

Cite this

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Single-Pair Courtship and Competition Assays in Drosophila

Abstract

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