@inproceedings{3ffbb01d513a4ff78ad16d0ba7720a5a,
title = "Single-molecule FRET experiments with a red-enhanced custom technology SPAD",
abstract = "Single-molecule fluorescence spectroscopy of freely diffusing molecules in solution is a powerful tool used to investigate the properties of individual molecules. Single-Photon Avalanche Diodes (SPADs) are the detectors of choice for these applications. Recently a new type of SPAD detector was introduced, dubbed red-enhanced SPAD (RE-SPAD), with good sensitivity throughout the visible spectrum and with excellent timing performance. We report a characterization of this new detector for single-molecule fluorescence resonant energy transfer (smFRET) studies on freely diffusing molecules in a confocal geometry and alternating laser excitation (ALEX) scheme. We use a series of doubly-labeled DNA molecules with donor-to-acceptor distances covering the whole range of useful FRET values. Both intensity-based (μs-ALEX) and lifetime-based (ns-ALEX) measurements are presented and compared to identical measurements performed with standard thick SPADs. Our results demonstrate the great potential of this new detector for smFRET measurements and beyond.",
keywords = "ALEX, FRET, SPAD, TCSPC, confocal, diffusion, lifetime, single-molecule",
author = "Francesco Panzeri and Antonino Ingargiola and Lin, {Ron R.} and Niusha Sarkhosh and Angelo Gulinatti and Ivan Rech and Massimo Ghioni and Sergio Cova and Shimon Weiss and Xavier Michalet",
year = "2013",
month = feb,
day = "2",
doi = "10.1117/12.2003187",
language = "אנגלית",
isbn = "9780819493590",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
booktitle = "Single Molecule Spectroscopy and Superresolution Imaging VI",
note = "Single Molecule Spectroscopy and Superresolution Imaging VI ; Conference date: 02-02-2013 Through 03-02-2013",
}