Single-cell RNA sequencing for the study of lupus nephritis

Evan Der; Hemant Suryawanshi; Jill Buyon; Thomas Tuschl; Chaim Putterman

doi:10.1136/lupus-2019-000329

Single-cell RNA sequencing for the study of lupus nephritis

Evan Der, Hemant Suryawanshi, Jill Buyon, Thomas Tuschl, Chaim Putterman

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Single-cell RNA sequencing (scRNA-seq) has recently undergone rapid advances in the development of this technology, leading to high throughput and accelerating discovery in many biological systems and diseases. The single-cell resolution of the technique allows for the investigation of heterogeneity in cell populations, and the pinpointing of pathological populations contributing to disease. Here we review the development of scRNA-seq technology and the analysis that has evolved with the ever-increasing throughput. Finally, we highlight recent applications of scRNA-seq to understand the molecular pathogenesis of lupus and lupus nephritis.

Original language	English
Article number	e000329
Journal	Lupus Science and Medicine
Volume	6
Issue number	1
DOIs	https://doi.org/10.1136/lupus-2019-000329
State	Published - 1 Jun 2019
Externally published	Yes

Bibliographical note

Publisher Copyright:
© Author(s) (or their employer(s)) 2018. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

Funding

Funding Funding was provided through grants from the National Institutes of Health (UH2-AR067676, UH2-AR067677, UH2-AR067679, UH2-AR067681, UH2-AR067685, UH2-AR067688, UH2-AR067689, UH2-AR067690, UH2-AR067691, UH2-AR067694, and UM2-AR067678). Competing interests None declared. Patient consent for publication Not required. Provenance and peer review Commissioned; externally peer reviewed. open access This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0

Funders	Funder number
National Institutes of Health	UH2-AR067677, UH2-AR067688, UH2-AR067689, UH2-AR067679, UH2-AR067685, UM2-AR067678, UH2-AR067676, UH2-AR067691, UH2-AR067681, UH2-AR067694, UH2-AR067690

Keywords

inflammation
lupus nephritis
systemic lupus erythematosus

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10.1136/lupus-2019-000329

Cite this

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title = "Single-cell RNA sequencing for the study of lupus nephritis",

abstract = "Single-cell RNA sequencing (scRNA-seq) has recently undergone rapid advances in the development of this technology, leading to high throughput and accelerating discovery in many biological systems and diseases. The single-cell resolution of the technique allows for the investigation of heterogeneity in cell populations, and the pinpointing of pathological populations contributing to disease. Here we review the development of scRNA-seq technology and the analysis that has evolved with the ever-increasing throughput. Finally, we highlight recent applications of scRNA-seq to understand the molecular pathogenesis of lupus and lupus nephritis.",

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AU - Suryawanshi, Hemant

AU - Buyon, Jill

AU - Tuschl, Thomas

AU - Putterman, Chaim

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Single-cell RNA sequencing for the study of lupus nephritis

Abstract

Bibliographical note

Funding

Keywords

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