Abstract
We generated a system for in vivo visualization and analysis of mammalian mRNA transcriptional kinetics of single alleles in real time, using single-gene integrations. We obtained high-resolution transcription measurements of a single cyclin D1 allele under endogenous or viral promoter control, including quantification of temporal kinetics of transcriptional bursting, promoter firing, nascent mRNA numbers and transcription rates during the cell cycle, and in relation to DNA replication.
| Original language | English |
|---|---|
| Pages (from-to) | 631-633 |
| Number of pages | 3 |
| Journal | Nature Methods |
| Volume | 7 |
| Issue number | 8 |
| DOIs | |
| State | Published - Aug 2010 |
Bibliographical note
Funding Information:We thank R. Pestell (Thomas Jefferson University) for the cyclin D1 promoter, C. Cardoso (Technische Universität Darmstadt) for RFP-PCNA and R. Drummer (Bar-Ilan University) for statistical analysis. This work was supported by grants to Y.S.-T. by the Israel Cancer Research Fund (Research Career Development Award), the Israel Ministry of Health, the Israel Cancer Association, the Alon Fellowship and the Jane Stern Lebell Family Fellowship of Bar-Ilan University; and to Y.S.-T. and Y.G. by the Israel Science Foundation Bikura grant. We thank the Israel Science Foundation for the fluorescence live-cell imaging microscopes.