Simplified riboprobe purification using translucent straws as gel tubes

Shahar Kol, Izhar Ben-Shlomo, Eli Y. Adashi, Richard M. Rohan

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Gel purification of radioactive riboprobes enhances the quality of the ribonuclease protection assay. A simple and effective method for riboprobe purification is described. The method uses acrylamide gels in plastic tubes to achieve electrophoretic separation of the RNA polymerase products.

Original languageEnglish
Pages (from-to)129-132
Number of pages4
JournalGenetic Analysis - Biomolecular Engineering
Volume12
Issue number3-4
DOIs
StatePublished - Jan 1996
Externally publishedYes

Bibliographical note

Funding Information:
* Corresponding author, 655 W. Baltimore Street, Room 11-007, Baltimore, Maryland, 21201. Tel.: 410 7064050; Fax: 410 7065747. ’ On leave from the Department of Obstetrics and Gynecology, Rambam Medical Center, Haifa, Israel. Recipient of an American Physician Fellowship, and of a 1994 Merck Senior Fellow Award of the Endocrine Society. * On leave from the Rebecca Sieff Hospital, Wed, Israel. Recipient of an International Fellowship Award from the Israeli Medical Association, a Lalor Foundation Fellowship Award and a Harlea Charitable Trust Award. 3 Supported in part by NIH Research Grants HD30288 and HD19998 (EYA).

Keywords

  • radioactive riboprobe
  • ribonuclease protection assay
  • riboprobe purification
  • translucent straw

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