Shedding light on mitochondrial function by real time monitoring of NADH fluorescence: I. Basic methodology and animal studies

Avraham Mayevsky, Efrat Barbiro-Michaely

Research output: Contribution to journalReview articlepeer-review

13 Scopus citations

Abstract

Normal mitochondrial function in the process of metabolic energy production is a key factor in maintaining cellular activities. Many pathological conditions in animals, as well as in patients, are directly or indirectly related to dysfunction of the mitochondria. Monitoring the mitochondrial activity by measuring the autofluorescence of NADH has been the most practical approach since the 1950s. This review presents the principles and technological aspects, as well as typical results, accumulated in our laboratory since the early 1970s. We were able to apply the fiber-optic-based NADH fluorometry to many organs monitored in vivo under various pathophysiological conditions in animals. These studies were the basis for the development of clinical monitoring devices as presented in accompanying article. The encouraging experimental results in animals stimulated us to apply the same technology in patients after technological adaptations as described in the accompanying article. Our medical device was approved for clinical use by the FDA.

Original languageEnglish
Pages (from-to)1-34
Number of pages34
JournalJournal of Clinical Monitoring and Computing
Volume27
Issue number1
DOIs
StatePublished - Feb 2013

Keywords

  • Brain metabolism
  • Cellular energy metabolism
  • In vivo monitoring
  • Intracellular NADH autofluorescence
  • Multiorgan monitoring
  • Tissue oxygen balance

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