TY - JOUR
T1 - Shedding light on mitochondrial function by real time monitoring of NADH fluorescence
T2 - II: Human studies
AU - Mayevsky, Avraham
AU - Barbiro-Michaely, Efrat
PY - 2013/4
Y1 - 2013/4
N2 - Monitoring the mitochondrial function, alone or together with microcirculatory blood flow, volume and hemoglobin oxygenation in patients, is very rare. The integrity of microcirculation and mitochondrial activity is a key factor in keeping normal cellular activities. Many pathological conditions in patients are directly or indirectly related to dysfunction of the mitochondria. Evaluation of mitochondrial activity by measuring the autofluorescence of NADH has been the most practical approach since the 1950s. This review, which accompanies part I, presents the principles and technological aspects of various devices used in order to monitor mitochondrial NADH redox state and tissue viability in patients. In part I, the detailed technological aspects of NADH monitoring were described. Typical results accumulated in our studies since the mid-1990s are presented as well. We were able to apply the fiber optic based NADH fluorometry to several organs monitored in vivo in patients under various pathophysiological conditions.
AB - Monitoring the mitochondrial function, alone or together with microcirculatory blood flow, volume and hemoglobin oxygenation in patients, is very rare. The integrity of microcirculation and mitochondrial activity is a key factor in keeping normal cellular activities. Many pathological conditions in patients are directly or indirectly related to dysfunction of the mitochondria. Evaluation of mitochondrial activity by measuring the autofluorescence of NADH has been the most practical approach since the 1950s. This review, which accompanies part I, presents the principles and technological aspects of various devices used in order to monitor mitochondrial NADH redox state and tissue viability in patients. In part I, the detailed technological aspects of NADH monitoring were described. Typical results accumulated in our studies since the mid-1990s are presented as well. We were able to apply the fiber optic based NADH fluorometry to several organs monitored in vivo in patients under various pathophysiological conditions.
KW - Brain metabolism
KW - Cellular energy metabolism
KW - In vivo monitoring
KW - Intracellular NADH autofluorescence
KW - Patients monitoring
KW - Tissue oxygen balance
UR - http://www.scopus.com/inward/record.url?scp=84879553974&partnerID=8YFLogxK
U2 - 10.1007/s10877-012-9413-6
DO - 10.1007/s10877-012-9413-6
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C2 - 23224276
AN - SCOPUS:84879553974
SN - 1387-1307
VL - 27
SP - 125
EP - 145
JO - Journal of Clinical Monitoring and Computing
JF - Journal of Clinical Monitoring and Computing
IS - 2
ER -