Separation of rare cell subpopulations with the aid of biotin-labelled ligands

Michael Steinitz, Alejandro Livoff, Sara Tamir, Talma Brenner

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


A universal method for selection of surface marker-positive cells is described. The cells, admixed with an excess of surface marker-negative cells, are In-st labelled with a specific biotinylated ligand and then isolated with the aid of monoclonal, anti-biotin coated beads. The method enables selection and isolation of cells with a frequency as low as 10-4. The ligand can be an antigen (for selection of infrequent antibody-producing cells), an antibody (for selection of surface antigen-positive cells) or other molecules (for selection of specific receptor-positive cells).

Original languageEnglish
Pages (from-to)49-52
Number of pages4
JournalMedical Oncology and Tumor Pharmacotherapy
Issue number1-2
StatePublished - Mar 1993
Externally publishedYes


  • Biotin
  • Human monoclonal antibodies
  • Rosette
  • Surface marker
  • lmmuno-selection


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