Abstract
A universal method for selection of surface marker-positive cells is described. The cells, admixed with an excess of surface marker-negative cells, are In-st labelled with a specific biotinylated ligand and then isolated with the aid of monoclonal, anti-biotin coated beads. The method enables selection and isolation of cells with a frequency as low as 10-4. The ligand can be an antigen (for selection of infrequent antibody-producing cells), an antibody (for selection of surface antigen-positive cells) or other molecules (for selection of specific receptor-positive cells).
Original language | English |
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Pages (from-to) | 49-52 |
Number of pages | 4 |
Journal | Medical Oncology and Tumor Pharmacotherapy |
Volume | 10 |
Issue number | 1-2 |
DOIs | |
State | Published - Mar 1993 |
Externally published | Yes |
Keywords
- Biotin
- Human monoclonal antibodies
- Rosette
- Surface marker
- lmmuno-selection