Sensitive Immunodetection of Severe Acute Respiratory Syndrome Coronavirus 2 Variants of Concern 501Y.V2 and 501Y.V1

Moria Barlev-Gross; Shay Weiss; Nir Paran; Yfat Yahalom-Ronen; Ofir Israeli; Ital Nemet; Limor Kliker; Neta Zuckerman; Itai Glinert; Tal Noy-Porat; Ron Alcalay; Ronit Rosenfeld; Haim Levy; Ohad Mazor; Michal Mandelboim; Ella Mendelson; Adi Beth-Din; Tomer Israely; Adva Mechaly

doi:10.1093/infdis/jiab278

Sensitive Immunodetection of Severe Acute Respiratory Syndrome Coronavirus 2 Variants of Concern 501Y.V2 and 501Y.V1

Moria Barlev-Gross
, Shay Weiss
, Nir Paran
, Yfat Yahalom-Ronen
, Ofir Israeli
, Ital Nemet
, Limor Kliker
, Neta Zuckerman
, Itai Glinert
, Tal Noy-Porat
, Ron Alcalay
, Ronit Rosenfeld
, Haim Levy
, Ohad Mazor
, Michal Mandelboim
, Ella Mendelson
, Adi Beth-Din
, Tomer Israely
, Adva Mechaly

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants may influence the effectiveness of existing laboratory diagnostics. In the current study we determined whether the British (20I/501Y.V1) and South African (20H/501Y.V2) SARS-CoV-2 variants of concern are detected with an in-house S1-based antigen detection assay, analyzing spiked pools of quantitative reverse-transcription polymerase chain reaction-negative nasopharyngeal swab specimens. The assay, combining 4 monoclonal antibodies, allowed sensitive detection of both the wild type and the variants of concern, despite accumulation of several mutations in the variants' S1 region - results suggesting that this combination, targeting distinct epitopes, enables both specificity and the universality.

Original language	English
Pages (from-to)	616-619
Number of pages	4
Journal	Journal of Infectious Diseases
Volume	224
Issue number	4
DOIs	https://doi.org/10.1093/infdis/jiab278
State	Published - 16 Aug 2021
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2021 The Author(s) 2021. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: [email protected].

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

501Y.V1
501Y.V2
Antibodies
Antigen detection
SARS-CoV-2
Variants of Concern
spike protein

Access to Document

10.1093/infdis/jiab278

Cite this

Barlev-Gross, M., Weiss, S., Paran, N., Yahalom-Ronen, Y., Israeli, O., Nemet, I., Kliker, L., Zuckerman, N., Glinert, I., Noy-Porat, T., Alcalay, R., Rosenfeld, R., Levy, H., Mazor, O., Mandelboim, M., Mendelson, E., Beth-Din, A., Israely, T., & Mechaly, A. (2021). Sensitive Immunodetection of Severe Acute Respiratory Syndrome Coronavirus 2 Variants of Concern 501Y.V2 and 501Y.V1. Journal of Infectious Diseases, 224(4), 616-619. https://doi.org/10.1093/infdis/jiab278

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abstract = "Emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants may influence the effectiveness of existing laboratory diagnostics. In the current study we determined whether the British (20I/501Y.V1) and South African (20H/501Y.V2) SARS-CoV-2 variants of concern are detected with an in-house S1-based antigen detection assay, analyzing spiked pools of quantitative reverse-transcription polymerase chain reaction-negative nasopharyngeal swab specimens. The assay, combining 4 monoclonal antibodies, allowed sensitive detection of both the wild type and the variants of concern, despite accumulation of several mutations in the variants' S1 region - results suggesting that this combination, targeting distinct epitopes, enables both specificity and the universality.",

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Barlev-Gross, M, Weiss, S, Paran, N, Yahalom-Ronen, Y, Israeli, O, Nemet, I, Kliker, L, Zuckerman, N, Glinert, I, Noy-Porat, T, Alcalay, R, Rosenfeld, R, Levy, H, Mazor, O, Mandelboim, M, Mendelson, E, Beth-Din, A, Israely, T & Mechaly, A 2021, 'Sensitive Immunodetection of Severe Acute Respiratory Syndrome Coronavirus 2 Variants of Concern 501Y.V2 and 501Y.V1', Journal of Infectious Diseases, vol. 224, no. 4, pp. 616-619. https://doi.org/10.1093/infdis/jiab278

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AU - Barlev-Gross, Moria

AU - Weiss, Shay

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AU - Alcalay, Ron

AU - Rosenfeld, Ronit

AU - Levy, Haim

AU - Mazor, Ohad

AU - Mandelboim, Michal

AU - Mendelson, Ella

AU - Beth-Din, Adi

AU - Israely, Tomer

AU - Mechaly, Adva

N1 - Publisher Copyright: © 2021 The Author(s) 2021. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: [email protected].

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Y1 - 2021/8/16

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AB - Emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants may influence the effectiveness of existing laboratory diagnostics. In the current study we determined whether the British (20I/501Y.V1) and South African (20H/501Y.V2) SARS-CoV-2 variants of concern are detected with an in-house S1-based antigen detection assay, analyzing spiked pools of quantitative reverse-transcription polymerase chain reaction-negative nasopharyngeal swab specimens. The assay, combining 4 monoclonal antibodies, allowed sensitive detection of both the wild type and the variants of concern, despite accumulation of several mutations in the variants' S1 region - results suggesting that this combination, targeting distinct epitopes, enables both specificity and the universality.

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Sensitive Immunodetection of Severe Acute Respiratory Syndrome Coronavirus 2 Variants of Concern 501Y.V2 and 501Y.V1

Abstract

Bibliographical note

UN SDGs

Keywords

Access to Document

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