Sensitive Immunodetection of Severe Acute Respiratory Syndrome Coronavirus 2 Variants of Concern 501Y.V2 and 501Y.V1

Moria Barlev-Gross; Shay Weiss; Nir Paran; Yfat Yahalom-Ronen; Ofir Israeli; Ital Nemet; Limor Kliker; Neta Zuckerman; Itai Glinert; Tal Noy-Porat; Ron Alcalay; Ronit Rosenfeld; Haim Levy; Ohad Mazor; Michal Mandelboim; Ella Mendelson; Adi Beth-Din; Tomer Israely; Adva Mechaly

doi:10.1093/infdis/jiab278

Sensitive Immunodetection of Severe Acute Respiratory Syndrome Coronavirus 2 Variants of Concern 501Y.V2 and 501Y.V1

Moria Barlev-Gross, Shay Weiss, Nir Paran, Yfat Yahalom-Ronen, Ofir Israeli, Ital Nemet, Limor Kliker, Neta Zuckerman, Itai Glinert, Tal Noy-Porat, Ron Alcalay, Ronit Rosenfeld, Haim Levy, Ohad Mazor, Michal Mandelboim, Ella Mendelson, Adi Beth-Din, Tomer Israely, Adva Mechaly

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants may influence the effectiveness of existing laboratory diagnostics. In the current study we determined whether the British (20I/501Y.V1) and South African (20H/501Y.V2) SARS-CoV-2 variants of concern are detected with an in-house S1-based antigen detection assay, analyzing spiked pools of quantitative reverse-transcription polymerase chain reaction-negative nasopharyngeal swab specimens. The assay, combining 4 monoclonal antibodies, allowed sensitive detection of both the wild type and the variants of concern, despite accumulation of several mutations in the variants' S1 region - results suggesting that this combination, targeting distinct epitopes, enables both specificity and the universality.

Original language	English
Pages (from-to)	616-619
Number of pages	4
Journal	Journal of Infectious Diseases
Volume	224
Issue number	4
DOIs	https://doi.org/10.1093/infdis/jiab278
State	Published - 16 Aug 2021
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2021 The Author(s) 2021. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: [email protected].

Keywords

501Y.V1
501Y.V2
Antibodies
Antigen detection
SARS-CoV-2
Variants of Concern
spike protein

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1093/infdis/jiab278

Cite this

Barlev-Gross, M., Weiss, S., Paran, N., Yahalom-Ronen, Y., Israeli, O., Nemet, I., Kliker, L., Zuckerman, N., Glinert, I., Noy-Porat, T., Alcalay, R., Rosenfeld, R., Levy, H., Mazor, O., Mandelboim, M., Mendelson, E., Beth-Din, A., Israely, T., & Mechaly, A. (2021). Sensitive Immunodetection of Severe Acute Respiratory Syndrome Coronavirus 2 Variants of Concern 501Y.V2 and 501Y.V1. Journal of Infectious Diseases, 224(4), 616-619. https://doi.org/10.1093/infdis/jiab278

@article{ae3282cbf72c4de2a74f6fa9a202ddf4,

title = "Sensitive Immunodetection of Severe Acute Respiratory Syndrome Coronavirus 2 Variants of Concern 501Y.V2 and 501Y.V1",

abstract = "Emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants may influence the effectiveness of existing laboratory diagnostics. In the current study we determined whether the British (20I/501Y.V1) and South African (20H/501Y.V2) SARS-CoV-2 variants of concern are detected with an in-house S1-based antigen detection assay, analyzing spiked pools of quantitative reverse-transcription polymerase chain reaction-negative nasopharyngeal swab specimens. The assay, combining 4 monoclonal antibodies, allowed sensitive detection of both the wild type and the variants of concern, despite accumulation of several mutations in the variants' S1 region - results suggesting that this combination, targeting distinct epitopes, enables both specificity and the universality.",

keywords = "501Y.V1, 501Y.V2, Antibodies, Antigen detection, SARS-CoV-2, Variants of Concern, spike protein",

author = "Moria Barlev-Gross and Shay Weiss and Nir Paran and Yfat Yahalom-Ronen and Ofir Israeli and Ital Nemet and Limor Kliker and Neta Zuckerman and Itai Glinert and Tal Noy-Porat and Ron Alcalay and Ronit Rosenfeld and Haim Levy and Ohad Mazor and Michal Mandelboim and Ella Mendelson and Adi Beth-Din and Tomer Israely and Adva Mechaly",

note = "Publisher Copyright: {\textcopyright} 2021 The Author(s) 2021. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: [email protected].",

year = "2021",

month = aug,

day = "16",

doi = "10.1093/infdis/jiab278",

language = "אנגלית",

volume = "224",

pages = "616--619",

journal = "Journal of Infectious Diseases",

issn = "0022-1899",

publisher = "Oxford University Press",

number = "4",

}

Barlev-Gross, M, Weiss, S, Paran, N, Yahalom-Ronen, Y, Israeli, O, Nemet, I, Kliker, L, Zuckerman, N, Glinert, I, Noy-Porat, T, Alcalay, R, Rosenfeld, R, Levy, H, Mazor, O, Mandelboim, M, Mendelson, E, Beth-Din, A, Israely, T & Mechaly, A 2021, 'Sensitive Immunodetection of Severe Acute Respiratory Syndrome Coronavirus 2 Variants of Concern 501Y.V2 and 501Y.V1', Journal of Infectious Diseases, vol. 224, no. 4, pp. 616-619. https://doi.org/10.1093/infdis/jiab278

TY - JOUR

T1 - Sensitive Immunodetection of Severe Acute Respiratory Syndrome Coronavirus 2 Variants of Concern 501Y.V2 and 501Y.V1

AU - Barlev-Gross, Moria

AU - Weiss, Shay

AU - Paran, Nir

AU - Yahalom-Ronen, Yfat

AU - Israeli, Ofir

AU - Nemet, Ital

AU - Kliker, Limor

AU - Zuckerman, Neta

AU - Glinert, Itai

AU - Noy-Porat, Tal

AU - Alcalay, Ron

AU - Rosenfeld, Ronit

AU - Levy, Haim

AU - Mazor, Ohad

AU - Mandelboim, Michal

AU - Mendelson, Ella

AU - Beth-Din, Adi

AU - Israely, Tomer

AU - Mechaly, Adva

N1 - Publisher Copyright: © 2021 The Author(s) 2021. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: [email protected].

PY - 2021/8/16

Y1 - 2021/8/16

N2 - Emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants may influence the effectiveness of existing laboratory diagnostics. In the current study we determined whether the British (20I/501Y.V1) and South African (20H/501Y.V2) SARS-CoV-2 variants of concern are detected with an in-house S1-based antigen detection assay, analyzing spiked pools of quantitative reverse-transcription polymerase chain reaction-negative nasopharyngeal swab specimens. The assay, combining 4 monoclonal antibodies, allowed sensitive detection of both the wild type and the variants of concern, despite accumulation of several mutations in the variants' S1 region - results suggesting that this combination, targeting distinct epitopes, enables both specificity and the universality.

AB - Emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants may influence the effectiveness of existing laboratory diagnostics. In the current study we determined whether the British (20I/501Y.V1) and South African (20H/501Y.V2) SARS-CoV-2 variants of concern are detected with an in-house S1-based antigen detection assay, analyzing spiked pools of quantitative reverse-transcription polymerase chain reaction-negative nasopharyngeal swab specimens. The assay, combining 4 monoclonal antibodies, allowed sensitive detection of both the wild type and the variants of concern, despite accumulation of several mutations in the variants' S1 region - results suggesting that this combination, targeting distinct epitopes, enables both specificity and the universality.

KW - 501Y.V1

KW - 501Y.V2

KW - Antibodies

KW - Antigen detection

KW - SARS-CoV-2

KW - Variants of Concern

KW - spike protein

UR - http://www.scopus.com/inward/record.url?scp=85114522395&partnerID=8YFLogxK

U2 - 10.1093/infdis/jiab278

DO - 10.1093/infdis/jiab278

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 34398244

AN - SCOPUS:85114522395

SN - 0022-1899

VL - 224

SP - 616

EP - 619

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

IS - 4

ER -

Sensitive Immunodetection of Severe Acute Respiratory Syndrome Coronavirus 2 Variants of Concern 501Y.V2 and 501Y.V1

Abstract

Bibliographical note

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this