Self-referencing digital holographic microscope for dynamic imaging of living cells

Arun Anand, Vani Chhaniwal, Swapnil Mahajan, Vismay Trivedi, Amardeep Singh, Rainer Leitgeb, Bahram Javidi

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

6 Scopus citations


Digital holographic microscope is an ideal tool for quantitative phase contrast imaging of living cells. It yields the thickness distribution of the object under investigation from a single hologram. From a series of holograms the dynamics of the cell under investigation can be obtained. But two-beam digital holographic microscopes has low temporal stability due to uncorrelated phase changes occurring in the reference and object arms. One way to overcome is to use common path techniques, in which, the reference beam is derived from the object beam itself. Both the beams travel along the same path, increasing the temporal stability of the setup. In self-referencing techniques a portion of the object beam is converted into reference beam. It could be achieved by example, using a glass plate to create two laterally sheared versions of the object beam at the sensor, which interfere to produce the holograms/interferograms. This created a common path setup, leading to high temporal stability (~0.6nm). This technique could be used to map cell membrane fluctuations with high temporal stability. Here we provide an overview of our work on the development of temporally stable quantitative phase contrast techniques for dynamic imaging of micro-objects and biological specimen including red blood cells.

Original languageEnglish
Title of host publicationThree-Dimensional Imaging, Visualization, and Display 2014
ISBN (Print)9781628410549
StatePublished - 2014
Externally publishedYes
EventThree-Dimensional Imaging, Visualization, and Display 2014 - Baltimore, MD, United States
Duration: 5 May 20147 May 2014

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X


ConferenceThree-Dimensional Imaging, Visualization, and Display 2014
Country/TerritoryUnited States
CityBaltimore, MD


FundersFunder number
University Grants Committee


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