TY - GEN
T1 - Self-referencing digital holographic microscope for dynamic imaging of living cells
AU - Anand, Arun
AU - Chhaniwal, Vani
AU - Mahajan, Swapnil
AU - Trivedi, Vismay
AU - Singh, Amardeep
AU - Leitgeb, Rainer
AU - Javidi, Bahram
PY - 2014
Y1 - 2014
N2 - Digital holographic microscope is an ideal tool for quantitative phase contrast imaging of living cells. It yields the thickness distribution of the object under investigation from a single hologram. From a series of holograms the dynamics of the cell under investigation can be obtained. But two-beam digital holographic microscopes has low temporal stability due to uncorrelated phase changes occurring in the reference and object arms. One way to overcome is to use common path techniques, in which, the reference beam is derived from the object beam itself. Both the beams travel along the same path, increasing the temporal stability of the setup. In self-referencing techniques a portion of the object beam is converted into reference beam. It could be achieved by example, using a glass plate to create two laterally sheared versions of the object beam at the sensor, which interfere to produce the holograms/interferograms. This created a common path setup, leading to high temporal stability (~0.6nm). This technique could be used to map cell membrane fluctuations with high temporal stability. Here we provide an overview of our work on the development of temporally stable quantitative phase contrast techniques for dynamic imaging of micro-objects and biological specimen including red blood cells.
AB - Digital holographic microscope is an ideal tool for quantitative phase contrast imaging of living cells. It yields the thickness distribution of the object under investigation from a single hologram. From a series of holograms the dynamics of the cell under investigation can be obtained. But two-beam digital holographic microscopes has low temporal stability due to uncorrelated phase changes occurring in the reference and object arms. One way to overcome is to use common path techniques, in which, the reference beam is derived from the object beam itself. Both the beams travel along the same path, increasing the temporal stability of the setup. In self-referencing techniques a portion of the object beam is converted into reference beam. It could be achieved by example, using a glass plate to create two laterally sheared versions of the object beam at the sensor, which interfere to produce the holograms/interferograms. This created a common path setup, leading to high temporal stability (~0.6nm). This technique could be used to map cell membrane fluctuations with high temporal stability. Here we provide an overview of our work on the development of temporally stable quantitative phase contrast techniques for dynamic imaging of micro-objects and biological specimen including red blood cells.
UR - http://www.scopus.com/inward/record.url?scp=84906343904&partnerID=8YFLogxK
U2 - 10.1117/12.2050415
DO - 10.1117/12.2050415
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AN - SCOPUS:84906343904
SN - 9781628410549
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Three-Dimensional Imaging, Visualization, and Display 2014
PB - SPIE
T2 - Three-Dimensional Imaging, Visualization, and Display 2014
Y2 - 5 May 2014 through 7 May 2014
ER -