Self-incompatibility related glycoproteins of Brassica are produced and secreted by transgenic tobacco cell cultures

Rafael Perl-Treves; Bruce Howlett; Mikhail Nasrallah

doi:10.1016/0168-9452(93)90070-g

Self-incompatibility related glycoproteins of Brassica are produced and secreted by transgenic tobacco cell cultures

Rafael Perl-Treves, Bruce Howlett, Mikhail Nasrallah

Cornell University

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A Nicotiana tabacum cell line was transformed, using particle bombardment, with constructs encoding the Brassica stigma glycoproteins, S-locus glycoprotein(SLG)-6 and S-locus related glycoprotein-1, under the control of a duplicated cauliflower mosaic virus 35S promoter and the nos terminator sequence. Calli and cell suspension lines that express each of the two proteins were recovered. Using specific antibodies, the foreign proteins can be detected in the cells, the cell wall eluate and the culture medium. The characteristic band patterns representing glycoforms (and isoforms) of the transgenically produced proteins on sodium dodecyl sulfate and isoelectric focusing protein gels are very similar to those from Brassica stigmas. Partial purification of SLG from the culture medium is described and the potential of the system for studying the biochemical basis of self-incompatibility is discussed.

Original language	English
Pages (from-to)	99-110
Number of pages	12
Journal	Plant Science
Volume	92
Issue number	1
DOIs	https://doi.org/10.1016/0168-9452(93)90070-g
State	Published - 1993
Externally published	Yes

Bibliographical note

Funding Information:
We wish to thank Rody Spivey and James Blankenship of the Plant Science Center at Cornell for their invaluable help in plant transformation procedures. We thank Dr. W. Crossby of the National Research Council, Canada, for providing the double-35S promoter. R.P.-T. was supported by a post-doctoral fellowship from the Yad Hanadiv-Rotschield Foundation, Jerusalem and M.E.N. was supported by a CIBA-GEIGY grant.

Funding

We wish to thank Rody Spivey and James Blankenship of the Plant Science Center at Cornell for their invaluable help in plant transformation procedures. We thank Dr. W. Crossby of the National Research Council, Canada, for providing the double-35S promoter. R.P.-T. was supported by a post-doctoral fellowship from the Yad Hanadiv-Rotschield Foundation, Jerusalem and M.E.N. was supported by a CIBA-GEIGY grant.

Funders	Funder number
CIBA-GEIGY
Yad Hanadiv-Rotschield Foundation

Keywords

Particle bombardment
Protein production
S-locus glycoprotein (SLG)
S-locus related glycoprotein-1 (SLR1)
Self-incompatibility
Tobacco cell culture

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10.1016/0168-9452(93)90070-g

Cite this

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title = "Self-incompatibility related glycoproteins of Brassica are produced and secreted by transgenic tobacco cell cultures",

abstract = "A Nicotiana tabacum cell line was transformed, using particle bombardment, with constructs encoding the Brassica stigma glycoproteins, S-locus glycoprotein(SLG)-6 and S-locus related glycoprotein-1, under the control of a duplicated cauliflower mosaic virus 35S promoter and the nos terminator sequence. Calli and cell suspension lines that express each of the two proteins were recovered. Using specific antibodies, the foreign proteins can be detected in the cells, the cell wall eluate and the culture medium. The characteristic band patterns representing glycoforms (and isoforms) of the transgenically produced proteins on sodium dodecyl sulfate and isoelectric focusing protein gels are very similar to those from Brassica stigmas. Partial purification of SLG from the culture medium is described and the potential of the system for studying the biochemical basis of self-incompatibility is discussed.",

keywords = "Particle bombardment, Protein production, S-locus glycoprotein (SLG), S-locus related glycoprotein-1 (SLR1), Self-incompatibility, Tobacco cell culture",

author = "Rafael Perl-Treves and Bruce Howlett and Mikhail Nasrallah",

note = "Funding Information: We wish to thank Rody Spivey and James Blankenship of the Plant Science Center at Cornell for their invaluable help in plant transformation procedures. We thank Dr. W. Crossby of the National Research Council, Canada, for providing the double-35S promoter. R.P.-T. was supported by a post-doctoral fellowship from the Yad Hanadiv-Rotschield Foundation, Jerusalem and M.E.N. was supported by a CIBA-GEIGY grant.",

year = "1993",

doi = "10.1016/0168-9452(93)90070-g",

language = "אנגלית",

volume = "92",

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journal = "Plant Science",

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T1 - Self-incompatibility related glycoproteins of Brassica are produced and secreted by transgenic tobacco cell cultures

AU - Perl-Treves, Rafael

AU - Howlett, Bruce

AU - Nasrallah, Mikhail

N1 - Funding Information: We wish to thank Rody Spivey and James Blankenship of the Plant Science Center at Cornell for their invaluable help in plant transformation procedures. We thank Dr. W. Crossby of the National Research Council, Canada, for providing the double-35S promoter. R.P.-T. was supported by a post-doctoral fellowship from the Yad Hanadiv-Rotschield Foundation, Jerusalem and M.E.N. was supported by a CIBA-GEIGY grant.

PY - 1993

Y1 - 1993

N2 - A Nicotiana tabacum cell line was transformed, using particle bombardment, with constructs encoding the Brassica stigma glycoproteins, S-locus glycoprotein(SLG)-6 and S-locus related glycoprotein-1, under the control of a duplicated cauliflower mosaic virus 35S promoter and the nos terminator sequence. Calli and cell suspension lines that express each of the two proteins were recovered. Using specific antibodies, the foreign proteins can be detected in the cells, the cell wall eluate and the culture medium. The characteristic band patterns representing glycoforms (and isoforms) of the transgenically produced proteins on sodium dodecyl sulfate and isoelectric focusing protein gels are very similar to those from Brassica stigmas. Partial purification of SLG from the culture medium is described and the potential of the system for studying the biochemical basis of self-incompatibility is discussed.

AB - A Nicotiana tabacum cell line was transformed, using particle bombardment, with constructs encoding the Brassica stigma glycoproteins, S-locus glycoprotein(SLG)-6 and S-locus related glycoprotein-1, under the control of a duplicated cauliflower mosaic virus 35S promoter and the nos terminator sequence. Calli and cell suspension lines that express each of the two proteins were recovered. Using specific antibodies, the foreign proteins can be detected in the cells, the cell wall eluate and the culture medium. The characteristic band patterns representing glycoforms (and isoforms) of the transgenically produced proteins on sodium dodecyl sulfate and isoelectric focusing protein gels are very similar to those from Brassica stigmas. Partial purification of SLG from the culture medium is described and the potential of the system for studying the biochemical basis of self-incompatibility is discussed.

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KW - S-locus related glycoprotein-1 (SLR1)

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Self-incompatibility related glycoproteins of Brassica are produced and secreted by transgenic tobacco cell cultures

Abstract

Bibliographical note

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Keywords

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