Selective inactivation of enzymes conjugated to nanoparticles using tuned laser illumination

Asaf Ilovitsh, Pazit Polak, Zeev Zalevsky, Orit Shefi

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


We report a novel method for specific deactivation of conjugated enzymes using laser-heated gold nanoparticles. Current methods involve treatment of the entire solution, thereby inactivating all bioactive components. Our method enables inactivation of only a single or subset of targeted enzymes. The selected enzyme is pre-conjugated to gold nanoparticles, which are specifically heated by a laser tuned to their surface plasmon resonance. We demonstrate inactivation of a selected enzyme, glucose oxidase, within a mixture of biomolecules. Illumination of non-conjugated enzymes and nanoparticles demonstrated specificity. We propose a novel method to quantitatively regulate enzyme activity, providing a building block for cellular and cell-free biochemical reactions.

Original languageEnglish
Pages (from-to)767-774
Number of pages8
JournalCytometry. Part A : the journal of the International Society for Analytical Cytology
Issue number8
StatePublished - Aug 2017

Bibliographical note

Publisher Copyright:
© 2016 International Society for Advancement of Cytometry


  • enzyme inactivation
  • laser heating
  • nanoparticles


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