Selective 17-β-estradiol molecular imprinting

Shimon Farber, Bernard S. Green, Abraham J. Domb

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


An efficient novel method for the synthesis of a covalent molecularly imprinted polymer (MIP) highly specific to β-estradiol have been developed. MIP prepared by both covalent and non covalent techniques, demonstrated high selectivity toward β-estradiol. MIPs were synthesized by radical polymerization of 17-β-estradiol 4-vinyl-benzene carboxyl or sulfonyl esters used as covalent functional monomers, methacrylic acid as noncovalent functional monomer, ethylene glycol dimethacrylate as crosslinking agent, and acetonitrile as swelling and porogenic component. Almost 35% (w/w) of 17-β-estradiol was successfully removed from the polymer network by basic hydrolysis. The binding ability of MIP was 10.73 μg/mg MIP following removal of 17-β-estradiol in the 2 mg/mL β-estradiol solution. Selective rebinding of β-estradiol toward MIP was tested in the presence of competitive binders including estrone, 19-nortestosterone, epiandrosterone, and cholesterol. Estrone having closest similar chemical structure to β-estradiol exhibited only 0.6 μg/mg MIP competitive binding, being exposed to equivalent concentrations. Moreover, other competitive steroids demonstrated negligible affinity toward MIP indicating high selectivity of novel MIP system toward β-estradiol.

Original languageEnglish
Pages (from-to)5534-5542
Number of pages9
JournalJournal of Polymer Science, Part A: Polymer Chemistry
Issue number20
StatePublished - 15 Oct 2009
Externally publishedYes


  • 17-β-estradiol
  • Crosslinking
  • Molecular imprinting
  • Radical polymerization


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