Screening for host factors directly interacting with RSV protein: Microfluidics

Sarit Kipper, Dorit Avrahami, Monika Bajorek, Doron Gerber

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

We present a high-throughput microfluidics platform to identify novel host cell binding partners of respiratory syncytial virus (RSV) matrix (M) protein. The device consists of thousands of reaction chambers controlled by micro-mechanical valves. The microfluidic device is mated to a microarray-printed custommade gene library. These genes are then transcribed and translated on-chip, resulting in a protein array ready for binding to RSV M protein. Even small viral proteome, such as that of RSV, presents a challenge due to the fact that viral proteins are usually multifunctional and thus their interaction with the host is complex. Protein microarrays technology allows the interrogation of protein-protein interactions, which could possibly overcome obstacles by using conventional high throughput methods. Using microfluidics platform we have identified new host interactors of M involved in various cellular pathways. A number of microfluidics based assays have already provided novel insights into the virus-host interactome, and the results have important implications for future antiviral strategies aimed at targets of viral protein interactions with the host.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages165-174
Number of pages10
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1442
ISSN (Print)1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2016.

Keywords

  • Integrated microfluidics
  • Protein arrays
  • RSV-host interaction
  • Virus-host interactions

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