S-phase transcriptional buffering quantified on two different promoters

Sharon Yunger, Pinhas Kafri, Liat Rosenfeld, Eliraz Greenberg, Noa Kinor, Yuval Garini, Yaron Shav-Tal

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Imaging of transcription by quantitative fluorescence-based techniques allows the examination of gene expression kinetics in single cells. Using a cell system for the in vivo visualization of mammalian mRNA transcriptional kinetics at single-gene resolution during the cell cycle, we previously demonstrated a reduction in transcription levels after replication. This phenomenon has been described as a homeostasis mechanism that buffers mRNA transcription levels with respect to the cell cycle stage and the number of transcribing alleles. Here, we examined how transcriptional buffering enforced during S phase affects two different promoters, the cytomegalovirus promoter versus the cyclin D1 promoter, that drive the same gene body. We found that global modulation of histone modifications could completely revert the transcription down-regulation imposed during replication. Furthermore, measuring these levels of transcriptional activity in fixed and living cells showed that the transcriptional potential of the genes was significantly higher than actual transcription levels, suggesting that promoters might normally be limited from reaching their full transcriptional potential.

Original languageEnglish
Article numbere201800086
Pages (from-to)e201800086
JournalLife Science Alliance
Issue number5
StatePublished - Oct 2018

Bibliographical note

Funding Information:
This work was supported by European Research Council (Y Shav-Tal) and the Israel Science Foundation No. 493/15 (Y Shav-Tal).

Publisher Copyright:
© 2018 Yunger et al.


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