@article{ffb82a0a53694056abe300637c805977,
title = "RTVP-1 regulates glioma cell migration and invasion via interaction with N-WASP and hnRNPK",
abstract = "Glioblastoma (GBM) are characterized by increased invasion into the surrounding normal brain tissue. RTVP-1 is highly expressed in GBM and regulates the migration and invasion of glioma cells. To further study RTVP-1 effects we performed a pull-down assay using His-tagged RTVP-1 followed by mass spectrometry and found that RTVP-1 was associated with the actin polymerization regulator, N-WASP. This association was further validated by co-immunoprecipitation and FRET analysis. We found that RTVP-1 increased cell spreading, migration and invasion and these effects were at least partly mediated by N-WASP. Another protein which was found by the pull-down assay to interact with RTVP-1 is hnRNPK. This protein has been recently reported to associate with and to inhibit the effect of N-WASP on cell spreading. hnRNPK decreased cell migration, spreading and invasion in glioma cells. Using co-immunoprecipitation we validated the interactions of hnRNPK with N-WASP and RTVP-1 in glioma cells. In addition, we found that overexpression of RTVP-1 decreased the association of N-WASP and hnRNPK. In summary, we report that RTVP-1 regulates glioma cell spreading, migration and invasion and that these effects are mediated via interaction with N-WASP and by interfering with the inhibitory effect of hnRNPK on the function of this protein.",
keywords = "Glioblastoma, Glioma cell migration, N-WASP, hnRNPK",
author = "Amotz Ziv-Av and Giladi, \{Nissim David\} and Lee, \{Hae Kyung\} and Simona Cazacu and Susan Finniss and Cunli Xiang and Pauker, \{Maor H.\} and Mira Barda-Saad and Laila Poisson and Chaya Brodie",
year = "2015",
month = aug,
day = "14",
doi = "10.18632/oncotarget.4471",
language = "אנגלית",
volume = "6",
pages = "19826--19840",
journal = "Oncotarget",
issn = "1949-2553",
publisher = "Impact Journals LLC",
number = "23",
}