Abstract
The authors monitored the fluorescence emission rate of single dye molecules on a dry surface with the near-field scanning optical microscope (NSOM). A Texas Red fluorophore was covalently attached to a short strand of a DNA molecule and bound to an amino-propyl-silanized glass coverslip. After imaging, the NSOM probe was stationed over a single molecule and its emission rate was recorded as function of time until its photodestruction. Counts were integrated for 5-20 ms and typical count rates were 5-30 kHz. A typical emission time trace is shown and described in detail.
| Original language | English |
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| Pages | 12-13 |
| Number of pages | 2 |
| State | Published - 1996 |
| Externally published | Yes |
| Event | Proceedings of the 1996 6th Quantum Electronics and Laser Science Conference, QELS - Anaheim, CA, USA Duration: 2 Jun 1996 → 7 Jun 1996 |
Conference
| Conference | Proceedings of the 1996 6th Quantum Electronics and Laser Science Conference, QELS |
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| City | Anaheim, CA, USA |
| Period | 2/06/96 → 7/06/96 |