Role of Taurine in Cellular Volume Regulation in Erythrocytes of Air-Breathing Catfish (Clarias magur) Under Osmotic Stress

Bodhisattwa Banerjee, Debaprasad Koner, Priyanka Lal, Suman Kumari, Rubaiya Hasan, Nirmalendu Saha

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The present study investigated the role of taurine in cellular volume regulation of erythrocytes isolated from freshwater air-breathing magur catfish (Clarias magur) under osmotic stress. Exposure of erythrocytes, pre-loaded with or without taurine, to hypotonic medium (− 80 mOsmol/L) led to a significant decrease in taurine level in the erythrocytes due to efflux of taurine through a band 3 transporter protein present in the plasma membrane with a slight increase in cellular volume of erythrocytes by 12–13%, whereas incubation of erythrocytes with hypertonic medium (+ 80 mOsmol/L) with taurine caused a significant uptake of taurine by the erythrocytes through the Na+-dependent pathway but without any loss of taurine from the erythrocytes which was accompanied by a slight decrease in the cellular volume of erythrocytes by 11–12%. Furthermore, a direct correlation between the osmosensitive cellular volume and taurine release could be established in the erythrocytes of magur catfish under hypotonic stress (r = 0.9921). In conclusion, the erythrocytes of air-breathing magur catfish do possess a very efficient taurine-dependent volume regulatory mechanism to resist the changes in cellular volume under anisotonic conditions as a unique adaptational strategy to defend against the osmosensitive changes in cellular volume of erythrocytes.

Original languageEnglish
Pages (from-to)1389-1397
Number of pages9
JournalProceedings of the National Academy of Sciences India Section B - Biological Sciences
Volume89
Issue number4
DOIs
StatePublished - 1 Dec 2019
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2019, The National Academy of Sciences, India.

Funding

This study was supported by a project sanctioned to the corresponding author by the Science and Engineering Research Board, New Delhi, and the DSA programme to the Department of Zoology, Shillong, by the University Grants Commission, New Delhi.

FundersFunder number
University Grants Commission
Science and Engineering Research Board

    Keywords

    • 4,4′-Di-isothiocyanatostilbene-2,2′-disulphonic acid
    • Anisotonicity
    • Band 3 protein transporter
    • Cellular water content
    • Hypertonicity
    • Hypotonicity

    Fingerprint

    Dive into the research topics of 'Role of Taurine in Cellular Volume Regulation in Erythrocytes of Air-Breathing Catfish (Clarias magur) Under Osmotic Stress'. Together they form a unique fingerprint.

    Cite this