TY - JOUR
T1 - Role of Adenosine Receptor Activation in Antioxidant Enzyme Regulation during Ischemia-Reperfusion in the Isolated Rat Heart
AU - Hochhauser, Edith
AU - Kaminski, Oleg
AU - Shalom, Hemdat
AU - Leshem, Dorit
AU - Shneyvays, Vladimir
AU - Shainberg, Asher
AU - Vidne, Bernardo A.
PY - 2004/4
Y1 - 2004/4
N2 - The aim of the present study was to investigate the protective role of pharmacological preconditioning on antioxidant enzymes using A1 and A3 adenosine receptor agonists in the recovery of the isolated myocardium after cardioplegic ischemia. Two different modes of preconditioning were studied: isolated rat hearts were perfused with A1 receptor agonist 2-chloro-N6-cyclopentyladenosine (CCPA) or A3 2-chloro-N6-(3-iodobenzyl) adenosine-5′-N-methyluronamide (Cl-IB-MECA) (1 nM), followed by cardioplegic ischemia and reperfusion (30 min each) (perfusion mode), or CCPA or Cl-IB-MECA (100 μg/kg) were injected intravenously 24 h before the experiment (injection mode). Hearts treated with CCPA improved in terms of mechanical function, infarct size, ATP levels, superoxide dismutase, and catalase (p < 0.005) in both modes of administration. Cl-IB-MECA was beneficial mainly in the injected group. Reduced damage to the mitochondria in the CCPA-treated hearts was observed using electron microscopy evaluation. In the Cl-IB-MECA-injected hearts, mitochondrial damage was moderate. CCPA in both modes of treatment and Cl-IB-MECA in the injected mode were beneficial in protecting the perfused isolated rat heart, subjected to normothermic cardioplegic ischemia. This protection was partially related to the higher myocardial activity of superoxide dismutase and catalase.
AB - The aim of the present study was to investigate the protective role of pharmacological preconditioning on antioxidant enzymes using A1 and A3 adenosine receptor agonists in the recovery of the isolated myocardium after cardioplegic ischemia. Two different modes of preconditioning were studied: isolated rat hearts were perfused with A1 receptor agonist 2-chloro-N6-cyclopentyladenosine (CCPA) or A3 2-chloro-N6-(3-iodobenzyl) adenosine-5′-N-methyluronamide (Cl-IB-MECA) (1 nM), followed by cardioplegic ischemia and reperfusion (30 min each) (perfusion mode), or CCPA or Cl-IB-MECA (100 μg/kg) were injected intravenously 24 h before the experiment (injection mode). Hearts treated with CCPA improved in terms of mechanical function, infarct size, ATP levels, superoxide dismutase, and catalase (p < 0.005) in both modes of administration. Cl-IB-MECA was beneficial mainly in the injected group. Reduced damage to the mitochondria in the CCPA-treated hearts was observed using electron microscopy evaluation. In the Cl-IB-MECA-injected hearts, mitochondrial damage was moderate. CCPA in both modes of treatment and Cl-IB-MECA in the injected mode were beneficial in protecting the perfused isolated rat heart, subjected to normothermic cardioplegic ischemia. This protection was partially related to the higher myocardial activity of superoxide dismutase and catalase.
UR - http://www.scopus.com/inward/record.url?scp=1542514723&partnerID=8YFLogxK
U2 - 10.1089/152308604322899404
DO - 10.1089/152308604322899404
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C2 - 15025935
AN - SCOPUS:1542514723
SN - 1523-0864
VL - 6
SP - 335
EP - 344
JO - Antioxidants and Redox Signaling
JF - Antioxidants and Redox Signaling
IS - 2
ER -