Abstract
During the early steps of transcription initiation, nascent RNAs stimulate transcriptional condensate formation, whereas the burst of RNAs produced during elongation stimulates condensate dissolution.
| Original language | English |
|---|---|
| Pages (from-to) | 207-225.e24 |
| Journal | Cell |
| Volume | 184 |
| Issue number | 1 |
| DOIs | |
| State | Published - 7 Jan 2021 |
| Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2020 Elsevier Inc.
Funding
We are grateful to Dora Tang and Ankur Jain for discussions regarding complex coacervates and their regulation by RNA. We also thank Mehdan Kardar and Young lab members for discussions; D. Reinberg, J.M. Calabrese, R. Jaenisch, and I. Cheeseman for reagents; W. Salmon of the W.M. Keck Microscopy Facility; and E. Diel of the Harvard Center for Biomedical Imaging. This work was supported by NIH grants GM123511 (to R.A.Y.), CA155258 (to R.A.Y.), 1F32CA254216-01 (to J.E.H.), and GM134734 (to I.I.C.); NSF grant PHY-1743900 (to A.K.C., R.A.Y., and P.A.S.); by the NSF-Simons Center for Mathematical and Statistical Analysis of Biology at Harvard (Award Number #1764269 ) and the Harvard Quantitative Biology Initiative (to K.S.); and the Gruss-Lipper postdoctoral fellowship and the Rothschild postdoctoral fellowship (to I.S.). We are grateful to Dora Tang and Ankur Jain for discussions regarding complex coacervates and their regulation by RNA. We also thank Mehdan Kardar and Young lab members for discussions; D. Reinberg, J.M. Calabrese, R. Jaenisch, and I. Cheeseman for reagents; W. Salmon of the W.M. Keck Microscopy Facility; and E. Diel of the Harvard Center for Biomedical Imaging. This work was supported by NIH grants GM123511 (to R.A.Y.), CA155258 (to R.A.Y.), 1F32CA254216-01 (to J.E.H.), and GM134734 (to I.I.C.); NSF grant PHY-1743900 (to A.K.C. R.A.Y. and P.A.S.); by the NSF-Simons Center for Mathematical and Statistical Analysis of Biology at Harvard (Award Number #1764269) and the Harvard Quantitative Biology Initiative (to K.S.); and the Gruss-Lipper postdoctoral fellowship and the Rothschild postdoctoral fellowship (to I.S.). Conceptualization, J.E.H. O.O. K.S. T.I.L. P.A.S. A.K.C. and R.A.Y.; Methodology, J.E.H. O.O. K.S. N.M.H. I.S. M.M.Z. J.O.A. A.V.Z. C.L. and A.K.C.; Software, J.E.H. and K.S.; Formal Analysis, J.E.H. O.O. and K.S.; Investigation, J.E.H. O.O. K.S. I.S. M.M.Z. J.O.A. and A.V.Z.; Resources, N.M.H. G.L. I.I.C. A.K.C. and R.A.Y.; Writing – Original Draft, J.E.H. O.O. K.S. A.K.C. and R.A.Y.; Visualization, J.E.H. O.O. and K.S.; Supervision, A.K.C. and R.A.Y.; Funding Acquisition, P.A.S. A.K.C. and R.A.Y. R.A.Y. is a founder and shareholder of Syros Pharmaceuticals, Camp4 Therapeutics, Omega Therapeutics, and Dewpoint Therapeutics. T.I.L. is a shareholder of Syros Pharmaceuticals and a consultant to Camp4 Therapeutics. A.K.C. and P.A.S. are shareholders and consultants to Dewpoint Therapeutics. A.K.C. is a SAB member of Omega Therapeutics. The Whitehead Institute has filed a patent application based on this study.
| Funders | Funder number |
|---|---|
| Camp4 Therapeutics | |
| Dewpoint Therapeutics | |
| Formal Analysis | |
| Harvard Center for Biomedical Imaging | |
| Harvard Quantitative Biology Initiative | |
| NSF-Simons | 1764269 |
| Omega Therapeutics | |
| Syros Pharmaceuticals | |
| National Science Foundation | PHY-1743900 |
| National Institutes of Health | GM123511, CA155258, 1F32CA254216-01, GM134734 |
| National Institute of General Medical Sciences | R01GM034277 |
| Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology |
Keywords
- RNA
- complex coacervates
- enhancer
- feedback
- mediator
- non-equilibrium
- noncoding RNA
- phase separation
- transcription
- transcriptional condensates