Abstract
During the early steps of transcription initiation, nascent RNAs stimulate transcriptional condensate formation, whereas the burst of RNAs produced during elongation stimulates condensate dissolution.
| Original language | English |
|---|---|
| Pages (from-to) | 207-225.e24 |
| Journal | Cell |
| Volume | 184 |
| Issue number | 1 |
| DOIs | |
| State | Published - 7 Jan 2021 |
| Externally published | Yes |
Bibliographical note
Funding Information:We are grateful to Dora Tang and Ankur Jain for discussions regarding complex coacervates and their regulation by RNA. We also thank Mehdan Kardar and Young lab members for discussions; D. Reinberg, J.M. Calabrese, R. Jaenisch, and I. Cheeseman for reagents; W. Salmon of the W.M. Keck Microscopy Facility; and E. Diel of the Harvard Center for Biomedical Imaging. This work was supported by NIH grants GM123511 (to R.A.Y.), CA155258 (to R.A.Y.), 1F32CA254216-01 (to J.E.H.), and GM134734 (to I.I.C.); NSF grant PHY-1743900 (to A.K.C., R.A.Y., and P.A.S.); by the NSF-Simons Center for Mathematical and Statistical Analysis of Biology at Harvard (Award Number #1764269 ) and the Harvard Quantitative Biology Initiative (to K.S.); and the Gruss-Lipper postdoctoral fellowship and the Rothschild postdoctoral fellowship (to I.S.).
Funding Information:
We are grateful to Dora Tang and Ankur Jain for discussions regarding complex coacervates and their regulation by RNA. We also thank Mehdan Kardar and Young lab members for discussions; D. Reinberg, J.M. Calabrese, R. Jaenisch, and I. Cheeseman for reagents; W. Salmon of the W.M. Keck Microscopy Facility; and E. Diel of the Harvard Center for Biomedical Imaging. This work was supported by NIH grants GM123511 (to R.A.Y.), CA155258 (to R.A.Y.), 1F32CA254216-01 (to J.E.H.), and GM134734 (to I.I.C.); NSF grant PHY-1743900 (to A.K.C. R.A.Y. and P.A.S.); by the NSF-Simons Center for Mathematical and Statistical Analysis of Biology at Harvard (Award Number #1764269) and the Harvard Quantitative Biology Initiative (to K.S.); and the Gruss-Lipper postdoctoral fellowship and the Rothschild postdoctoral fellowship (to I.S.). Conceptualization, J.E.H. O.O. K.S. T.I.L. P.A.S. A.K.C. and R.A.Y.; Methodology, J.E.H. O.O. K.S. N.M.H. I.S. M.M.Z. J.O.A. A.V.Z. C.L. and A.K.C.; Software, J.E.H. and K.S.; Formal Analysis, J.E.H. O.O. and K.S.; Investigation, J.E.H. O.O. K.S. I.S. M.M.Z. J.O.A. and A.V.Z.; Resources, N.M.H. G.L. I.I.C. A.K.C. and R.A.Y.; Writing – Original Draft, J.E.H. O.O. K.S. A.K.C. and R.A.Y.; Visualization, J.E.H. O.O. and K.S.; Supervision, A.K.C. and R.A.Y.; Funding Acquisition, P.A.S. A.K.C. and R.A.Y. R.A.Y. is a founder and shareholder of Syros Pharmaceuticals, Camp4 Therapeutics, Omega Therapeutics, and Dewpoint Therapeutics. T.I.L. is a shareholder of Syros Pharmaceuticals and a consultant to Camp4 Therapeutics. A.K.C. and P.A.S. are shareholders and consultants to Dewpoint Therapeutics. A.K.C. is a SAB member of Omega Therapeutics. The Whitehead Institute has filed a patent application based on this study.
Publisher Copyright:
© 2020 Elsevier Inc.
Keywords
- RNA
- complex coacervates
- enhancer
- feedback
- mediator
- non-equilibrium
- noncoding RNA
- phase separation
- transcription
- transcriptional condensates