Retinoic acid synthesis by ALDH1A proteins is dispensable for meiosis initiation in the mouse fetal ovary

Anne Amandine Chassot, Morgane Le Rolle, Geneviève Jolivet, Isabelle Stevant, Jean Marie Guigonis, Jean Marie Guigonis, Fabio Da Silva, Fabio Da Silva, Serge Nef, Eric Pailhoux, Andreas Schedl, Norbert B. Ghyselinck, Marie Christine Chaboissier

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

In mammals, the timing of meiosis entry is regulated by signals from the gonadal environment. All-trans retinoic acid (ATRA) signaling is considered the key pathway that promotes Stra8 (stimulated by retinoic acid 8) expression and, in turn, meiosis entry. This model, however, is debated because it is based on analyzing the effects of exogenous ATRA on ex vivo gonadal cultures, which not accurately reflects the role of endogenous ATRA. Aldh1a1 and Aldh1a2, two retinaldehyde dehydrogenases synthesizing ATRA, are expressed in the mouse ovaries when meiosis initiates. Contrary to the present view, here, we demonstrate that ATRA-responsive cells are scarce in the ovary. Using three distinct gene deletion models for Aldh1a1;Aldh1a2;Aldh1a3, we show that Stra8 expression is independent of ATRA production by ALDH1A proteins and that germ cells progress through meiosis. Together, these data demonstrate that ATRA signaling is dispensable for instructing meiosis initiation in female germ cells.

Original languageEnglish
Article numberEAAZ1261
JournalScience advances
Volume6
Issue number21
DOIs
StatePublished - May 2020
Externally publishedYes

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