TY - JOUR
T1 - Retinoic acid synthesis by ALDH1A proteins is dispensable for meiosis initiation in the mouse fetal ovary
AU - Chassot, Anne Amandine
AU - Rolle, Morgane Le
AU - Jolivet, Geneviève
AU - Stevant, Isabelle
AU - Guigonis, Jean Marie
AU - Guigonis, Jean Marie
AU - Silva, Fabio Da
AU - Silva, Fabio Da
AU - Nef, Serge
AU - Pailhoux, Eric
AU - Schedl, Andreas
AU - Ghyselinck, Norbert B.
AU - Chaboissier, Marie Christine
N1 - Publisher Copyright:
© 2020 The Authors.
PY - 2020/5
Y1 - 2020/5
N2 - In mammals, the timing of meiosis entry is regulated by signals from the gonadal environment. All-trans retinoic acid (ATRA) signaling is considered the key pathway that promotes Stra8 (stimulated by retinoic acid 8) expression and, in turn, meiosis entry. This model, however, is debated because it is based on analyzing the effects of exogenous ATRA on ex vivo gonadal cultures, which not accurately reflects the role of endogenous ATRA. Aldh1a1 and Aldh1a2, two retinaldehyde dehydrogenases synthesizing ATRA, are expressed in the mouse ovaries when meiosis initiates. Contrary to the present view, here, we demonstrate that ATRA-responsive cells are scarce in the ovary. Using three distinct gene deletion models for Aldh1a1;Aldh1a2;Aldh1a3, we show that Stra8 expression is independent of ATRA production by ALDH1A proteins and that germ cells progress through meiosis. Together, these data demonstrate that ATRA signaling is dispensable for instructing meiosis initiation in female germ cells.
AB - In mammals, the timing of meiosis entry is regulated by signals from the gonadal environment. All-trans retinoic acid (ATRA) signaling is considered the key pathway that promotes Stra8 (stimulated by retinoic acid 8) expression and, in turn, meiosis entry. This model, however, is debated because it is based on analyzing the effects of exogenous ATRA on ex vivo gonadal cultures, which not accurately reflects the role of endogenous ATRA. Aldh1a1 and Aldh1a2, two retinaldehyde dehydrogenases synthesizing ATRA, are expressed in the mouse ovaries when meiosis initiates. Contrary to the present view, here, we demonstrate that ATRA-responsive cells are scarce in the ovary. Using three distinct gene deletion models for Aldh1a1;Aldh1a2;Aldh1a3, we show that Stra8 expression is independent of ATRA production by ALDH1A proteins and that germ cells progress through meiosis. Together, these data demonstrate that ATRA signaling is dispensable for instructing meiosis initiation in female germ cells.
UR - http://www.scopus.com/inward/record.url?scp=85086008137&partnerID=8YFLogxK
U2 - 10.1126/sciadv.aaz1261
DO - 10.1126/sciadv.aaz1261
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C2 - 32494737
AN - SCOPUS:85086008137
SN - 2375-2548
VL - 6
JO - Science advances
JF - Science advances
IS - 21
M1 - EAAZ1261
ER -