Responses of rat brain to induced spreading depression following exposure to carbon monoxide

Sigal Meilin, Judith Sonn, Nili Zarchin, Gennady Rogatsky, Esther Guggenheimer-Furman, Avraham Mayevsky

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    8 Scopus citations

    Abstract

    Until recently carbon monoxide (CO) was known only for its noxious effects. Exposure to CO results in an autoregulatory increase in cerebral blood flow (CBF). Little information is available on brain energy metabolism under low CO concentrations and on the effect of CO on the stimulated brain. In this study cortical spreading depression (SD) was induced in order to cause transient brain depolarization and increased energy demand. The multisite assembly (MSA), which contains four bundles of optical fibers for monitoring the intramitochondrial NADH redox state and tissue reflectance as well as four DC electrodes enabling measurement from four consecutive points on the cerebral cortex, was used to measure energy metabolism and the propagation of SD waves during exposure to CO. CBF in the contralateral hemisphere was measured using the laser Doppler technique. Three experimental groups of animals were examined: SD was induced during exposure to 1000 ppm CO, immediately after exposure to CO and 90 min after cessation of exposure to CO. Three control groups were also examined, in which the animals underwent the same procedures but were not exposed to CO. In all animals exposure to CO was followed by a significant increase in CBF. The greatest effect was found when SD was induced immediately after cessation of exposure to CO. SD wave frequency decreased when induced immediately after exposure to CO, whereas it increased when SD was induced 90 min after exposure. The amplitude of the NADH oxidation waves and their integral were smaller during SD induced immediately after exposure to CO. The DC potential did not change, suggesting that CO did not affect the SD initiation mechanism but rather resulted in energy depletion during recovery from SD. This study demonstrates that even at a concentration of 1000 ppm CO interferes with the metabolic activity of the brain during repolarization of the SD-induced negativity.

    Original languageEnglish
    Pages (from-to)323-328
    Number of pages6
    JournalBrain Research
    Volume780
    Issue number2
    DOIs
    StatePublished - 12 Jan 1998

    Bibliographical note

    Funding Information:
    This work was supported by a grant from the Committee for Research and Prevention in Occupational Safety and Health, Ministry of Labour and Social Affairs, Israel, and by the Chief Scientist's Office, Ministry of Health, Israel.

    Keywords

    • Carbon monoxide
    • Cortical spreading depression
    • DC potential
    • Energy metabolism
    • Mitochondrial NADH

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