Resistance of hepatitis delta virus replication to interferon-α treatment in transfected human cells

Y. Ilan, A. Klein, J. Taylor, R. Tur-Kaspa

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Interferon-α (IFN-α) is known to inhibit both DNA and RNA viruses, including hepatitis B virus (HBV). In humans the antiviral effect, if any, of IFN-α on hepatitis delta virus (HDV) is complicated by the fact that HDV is spread only to patients already infected with HBV. An in vitro model system was used to assay for an antiviral effect of IFN-α on HDV genome replication. Hep G2 cells were transfected with a plasmid containing a trimer of HDV and treated with IFN (20 or 100 units/mL) starting 1-7 days after transfection. RNA extracted from treated and nontreated cells was assayed by both slot blot and Northern analyses. The IFN-α treatment as expected increased the 2′-5′ oligo A synthetase RNA activity, but it did not affect HDV genome replication. Thus, in the absence of HBV, it appears that HDV is resistant to IFN-α.

Original languageEnglish
Pages (from-to)1164-1166
Number of pages3
JournalJournal of Infectious Diseases
Volume166
Issue number5
DOIs
StatePublished - Nov 1992
Externally publishedYes

Bibliographical note

Funding Information:
Received II March 1992; revised 3 June 1992. Grant support: Binational Science Foundation of the United States and Israel (to R.T.K.). Reprints or correspondence: R. Tur-Kaspa, Division ofMedicine. Hadassah University Hospital. 91120 Jerusalem. Israel.

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