Reproducible strategy for excisional skin-wound-healing studies in mice

Matan Yampolsky, Ido Bachelet, Yaron Fuchs

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Wound healing is a complex physiological process involving various cell types and signaling pathways. The capability to observe the dynamics of wound repair offers valuable insights into the effects of genetic modifications, pharmaceutical interventions or other experimental manipulations on the skin-repair process. Here, we provide a comprehensive protocol for a full-thickness, excisional skin-wound-healing assay in mice, which can easily be performed by any scientist who has received an animal welfare course certificate and can be completed within ~3 h, depending on the number of animals. Crucially, we highlight the importance of considering key aspects of the assay that can dramatically contribute to the reliability and reproducibility of these experiments. We thoroughly discuss the experimental design, necessary preparations, wounding technique and analysis. In addition, we discuss the use of lineage-tracing techniques to monitor cell migration, differentiation and the contribution of different cell populations to the repair process. Overall, we explore key aspects of the skin-wound-healing assay, supplying a detailed procedure and guidelines essential for decreasing variability and obtaining reliable and reproducible results.

Original languageEnglish
Pages (from-to)184-206
Number of pages23
JournalNature Protocols
Volume19
Issue number1
DOIs
StatePublished - Jan 2024
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2023, Springer Nature Limited.

Funding

We thank current and former members of the Fuchs group for their insights and experience. We thank M. Yosopuva, R. Ankawa and N. Goldberger, for their technical assistance; V. Zlobin and Y. Nissan for animal house support; and N. Dahan and Y. Lupu-haber for insights regarding microscopy. We extend special thanks to D. Kulinsky and A. Hezi-Yamit for valuable input. Y.F. is supported by the EMBO Young Investigator program.

FundersFunder number
European Molecular Biology Organization

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