Replacing a Century Old Technique-Modern Spectroscopy Can Supplant Gram Staining

Shirly Berezin; Yaron Aviv; Hagit Aviv; Elad Goldberg; Yaakov R. Tischler

doi:10.1038/s41598-017-02212-2

Replacing a Century Old Technique-Modern Spectroscopy Can Supplant Gram Staining

Shirly Berezin, Yaron Aviv, Hagit Aviv, Elad Goldberg, Yaakov R. Tischler

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Rapid and accurate Gram differentiation is paramount as the first step of pathogen identification and antibiotics administration. However, the current method requires additional reagents, is time-consuming, and is operator dependent. Here we show the principle of tip enhanced Raman spectroscopy (TERS) can differentiate between Gram negative and positive species, by detecting the changes in tip-enhancement in the Raman scattering from the bacteria's lipid-bilayer membrane, which specifically enhances Gram negative bacteria.

Original language	English
Article number	3810
Journal	Scientific Reports
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s41598-017-02212-2
State	Published - 19 Jun 2017

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© 2017 The Author(s).

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10.1038/s41598-017-02212-2

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abstract = "Rapid and accurate Gram differentiation is paramount as the first step of pathogen identification and antibiotics administration. However, the current method requires additional reagents, is time-consuming, and is operator dependent. Here we show the principle of tip enhanced Raman spectroscopy (TERS) can differentiate between Gram negative and positive species, by detecting the changes in tip-enhancement in the Raman scattering from the bacteria's lipid-bilayer membrane, which specifically enhances Gram negative bacteria.",

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Replacing a Century Old Technique-Modern Spectroscopy Can Supplant Gram Staining

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