Renal ischemia - Reperfusion injury: Contribution of nitric oxide and renal blood flow

Elisheva Mashiach, Shifra Sela, Josi Winaver, Shaul M. Shasha, Batya Kristal

Research output: Contribution to journalArticlepeer-review

41 Scopus citations


The contributions of nitric oxide (NO) and renal blood flow (RBF) were examined in ischemia-reperfusion injury in the rat kidney. The function of both kidneys was assessed by glomerular filtration rate (GFR), and fractional excretion of sodium (FE(Na)), calculated before, during unilateral renal artery clamping (45 min), and following reperfusion (90 min). RBF was measured in the same model by ultrasonic flowmetry. Intrarenal NO levels were modulated by administration of S-nitroso-N-acetylpenicillamine (SNAP), L-arginine, acetylcholine, and the NO synthase inhibitor N(G)-nitro-L-arginine methyl ester (L-NAME). SNAP increased GFR from 0.20 ± 0.04 ml/min in control ischemic kidney to 0.38 ± 0.06 ml/min and reduced FE(Na) from 19.3 ± 3.4 to 9.5 ± 1.8%. Similar results were observed when L-arginine was administered. Acetylcholine had no effect on GFR or FE(Na). RBF was fully restored within 60 min following reperfusion, with no change in the rate of recovery by L-arginine. L-NAME aggravated the ischemia-reperfusion injury, preventing full restoration of RBF, further reducing GFR and worsening FE(Na). In conclusion, ischemia-reperfusion injury ends in low intrarenal levels of NO. We propose that this low NO level results from damage to the endothelial receptor signal transduction process and is not due to impaired NO synthase activity or to changes in RBF.

Original languageEnglish
Pages (from-to)458-467
Number of pages10
Issue number4
StatePublished - Dec 1998
Externally publishedYes


  • Acetylcholine
  • L-arginine
  • N(G)-nitro-L-arginine methyl ester
  • Nitric oxide
  • Renal blood flow
  • Renal ischemia
  • Reperfusion
  • S-nitroso-N-acetylpenicillamine


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