Relative intensities of recognition factors at two combining sites of Ralstonia solanacearum lectin (RSL) for accommodating lFucα1→, dManα1→ and Galβ1→3/4GlcNAc glycotopes

Albert M. Wu; Jia Hua Liu; Anthony Herp; Dvora Sudakevitz; Nechama Gilboa-Garber

doi:10.1016/j.febslet.2012.03.024

Relative intensities of recognition factors at two combining sites of Ralstonia solanacearum lectin (RSL) for accommodating lFucα1→, dManα1→ and Galβ1→3/4GlcNAc glycotopes

Albert M. Wu
, Jia Hua Liu
, Anthony Herp
, Dvora Sudakevitz
, Nechama Gilboa-Garber

The Mina and Everard Goodman Faculty of Life Sciences - at Bar-Ilan University

Chang Gung University
Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Owing to the weak reactivities of monomeric dManα1 and Galβ1→3/4GlcNAcβ (I_β/II_β) glycotopes with Ralstonia solanacearum lectin (RSL), their recognition roles were previously ignored. In this study, the interaction intensities of RSL toward four monomeric glycotopes lFucα1→, dManα1→ and I_β/II_β within two combining sites were established by both enzyme-linked lectinosorbent and inhibition assays. It was found that high density of lFucα1→ complex enhanced the recognition intensities at lFucα1→ site, polyvalent dManα1→ was essential for binding at the dManα1→ site and polyvalent I _β/II_β was required at lFucα1→ site. The peculiar recognition systems of RSL are very different from other well known microbial lectins.

Original language	English
Pages (from-to)	1294-1299
Number of pages	6
Journal	FEBS Letters
Volume	586
Issue number	9
DOIs	https://doi.org/10.1016/j.febslet.2012.03.024
State	Published - 7 May 2012

Bibliographical note

Funding Information:
This work was supported by Grants from the Chang-Gung Medical Research Project (CMRP No. 180482 and 170443), Kwei-san, Tao-yuan, Taiwan, the National Science Council (NSC 100-2320-B-182-027 and 100-2320-B-182-028), Taipei, Taiwan. The authors thank for Ms. Yu Ping Gong’s technical assistance and data collection.

Funding

This work was supported by Grants from the Chang-Gung Medical Research Project (CMRP No. 180482 and 170443), Kwei-san, Tao-yuan, Taiwan, the National Science Council (NSC 100-2320-B-182-027 and 100-2320-B-182-028), Taipei, Taiwan. The authors thank for Ms. Yu Ping Gong’s technical assistance and data collection.

Funders	Funder number
CMRP	170443, 180482
Chang-Gung Medical Research Project
National Science Council	100-2320-B-182-028, 100-2320-B-182-027

Keywords

ABH active O-glycans
Carbohydrate recognition domain
Mannan
Ralstonia solanacearum lectin

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10.1016/j.febslet.2012.03.024

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title = "Relative intensities of recognition factors at two combining sites of Ralstonia solanacearum lectin (RSL) for accommodating lFucα1→, dManα1→ and Galβ1→3/4GlcNAc glycotopes",

abstract = "Owing to the weak reactivities of monomeric dManα1 and Galβ1→3/4GlcNAcβ (Iβ/IIβ) glycotopes with Ralstonia solanacearum lectin (RSL), their recognition roles were previously ignored. In this study, the interaction intensities of RSL toward four monomeric glycotopes lFucα1→, dManα1→ and Iβ/IIβ within two combining sites were established by both enzyme-linked lectinosorbent and inhibition assays. It was found that high density of lFucα1→ complex enhanced the recognition intensities at lFucα1→ site, polyvalent dManα1→ was essential for binding at the dManα1→ site and polyvalent I β/IIβ was required at lFucα1→ site. The peculiar recognition systems of RSL are very different from other well known microbial lectins.",

keywords = "ABH active O-glycans, Carbohydrate recognition domain, Mannan, Ralstonia solanacearum lectin",

author = "Wu, \{Albert M.\} and Liu, \{Jia Hua\} and Anthony Herp and Dvora Sudakevitz and Nechama Gilboa-Garber",

note = "Funding Information: This work was supported by Grants from the Chang-Gung Medical Research Project (CMRP No. 180482 and 170443), Kwei-san, Tao-yuan, Taiwan, the National Science Council (NSC 100-2320-B-182-027 and 100-2320-B-182-028), Taipei, Taiwan. The authors thank for Ms. Yu Ping Gong{\textquoteright}s technical assistance and data collection.",

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AU - Wu, Albert M.

AU - Liu, Jia Hua

AU - Herp, Anthony

AU - Sudakevitz, Dvora

AU - Gilboa-Garber, Nechama

N1 - Funding Information: This work was supported by Grants from the Chang-Gung Medical Research Project (CMRP No. 180482 and 170443), Kwei-san, Tao-yuan, Taiwan, the National Science Council (NSC 100-2320-B-182-027 and 100-2320-B-182-028), Taipei, Taiwan. The authors thank for Ms. Yu Ping Gong’s technical assistance and data collection.

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N2 - Owing to the weak reactivities of monomeric dManα1 and Galβ1→3/4GlcNAcβ (Iβ/IIβ) glycotopes with Ralstonia solanacearum lectin (RSL), their recognition roles were previously ignored. In this study, the interaction intensities of RSL toward four monomeric glycotopes lFucα1→, dManα1→ and Iβ/IIβ within two combining sites were established by both enzyme-linked lectinosorbent and inhibition assays. It was found that high density of lFucα1→ complex enhanced the recognition intensities at lFucα1→ site, polyvalent dManα1→ was essential for binding at the dManα1→ site and polyvalent I β/IIβ was required at lFucα1→ site. The peculiar recognition systems of RSL are very different from other well known microbial lectins.

AB - Owing to the weak reactivities of monomeric dManα1 and Galβ1→3/4GlcNAcβ (Iβ/IIβ) glycotopes with Ralstonia solanacearum lectin (RSL), their recognition roles were previously ignored. In this study, the interaction intensities of RSL toward four monomeric glycotopes lFucα1→, dManα1→ and Iβ/IIβ within two combining sites were established by both enzyme-linked lectinosorbent and inhibition assays. It was found that high density of lFucα1→ complex enhanced the recognition intensities at lFucα1→ site, polyvalent dManα1→ was essential for binding at the dManα1→ site and polyvalent I β/IIβ was required at lFucα1→ site. The peculiar recognition systems of RSL are very different from other well known microbial lectins.

KW - ABH active O-glycans

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KW - Ralstonia solanacearum lectin

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Relative intensities of recognition factors at two combining sites of Ralstonia solanacearum lectin (RSL) for accommodating lFucα1→, dManα1→ and Galβ1→3/4GlcNAc glycotopes

Abstract

Bibliographical note

Funding

Keywords

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