Relationship between the Expression Level of PSMD11 and Other Proteasome Proteins with the Activity of Ricin and Viscumin

D. V. Maltseva, M. P. Raigorodskaya, O. V. Tikhonova, E. N. Knyazev, E. A. Tonevitsky

Research output: Contribution to journalArticlepeer-review

Abstract

Abstract –: The role of proteasome proteins and proteins of the ERAD system in the cytotoxicity of type II ribosome-inactivating proteins ricin and viscumin was investigated. For this, the cell line of colorectal adenocarcinoma HT29, as well as the HT29-sh002 line obtained on its basis, were used. On the basis on the proteome analysis of these lines and the estimation of the proportion of inactivated ribosomes, it was shown that the contribution of the proteasome to the degradation of the catalytic subunits of toxins is different. The role of the Cdc37 co-chaperone in maintaining the stability of A subunit of viscumin in the cytoplasm is shown.

Original languageEnglish
Pages (from-to)198-200
Number of pages3
JournalDoklady Biochemistry and Biophysics
Volume493
Issue number1
DOIs
StatePublished - 1 Jul 2020
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2020, Pleiades Publishing, Ltd.

Funding

This work was supported by the Russian Science Foundation (project no. 17-14-01338).

FundersFunder number
Russian Science Foundation17-14-01338

    Keywords

    • Cdc37
    • ERAD
    • HT29
    • MLI
    • PSMD11
    • degradation
    • proteasome
    • ribosome inactivating protein
    • ricin
    • viscumin

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