Relationship between the Expression Level of PSMD11 and Other Proteasome Proteins with the Activity of Ricin and Viscumin

D. V. Maltseva; M. P. Raigorodskaya; O. V. Tikhonova; E. N. Knyazev; E. A. Tonevitsky

doi:10.1134/s1607672920040080

Relationship between the Expression Level of PSMD11 and Other Proteasome Proteins with the Activity of Ricin and Viscumin

D. V. Maltseva, M. P. Raigorodskaya, O. V. Tikhonova, E. N. Knyazev, E. A. Tonevitsky

Research output: Contribution to journal › Article › peer-review

Abstract

Abstract –: The role of proteasome proteins and proteins of the ERAD system in the cytotoxicity of type II ribosome-inactivating proteins ricin and viscumin was investigated. For this, the cell line of colorectal adenocarcinoma HT29, as well as the HT29-sh002 line obtained on its basis, were used. On the basis on the proteome analysis of these lines and the estimation of the proportion of inactivated ribosomes, it was shown that the contribution of the proteasome to the degradation of the catalytic subunits of toxins is different. The role of the Cdc37 co-chaperone in maintaining the stability of A subunit of viscumin in the cytoplasm is shown.

Original language	English
Pages (from-to)	198-200
Number of pages	3
Journal	Doklady Biochemistry and Biophysics
Volume	493
Issue number	1
DOIs	https://doi.org/10.1134/s1607672920040080
State	Published - 1 Jul 2020
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2020, Pleiades Publishing, Ltd.

Funding

This work was supported by the Russian Science Foundation (project no. 17-14-01338).

Funders	Funder number
Russian Science Foundation	17-14-01338

Keywords

Cdc37
ERAD
HT29
MLI
PSMD11
degradation
proteasome
ribosome inactivating protein
ricin
viscumin

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abstract = "Abstract –: The role of proteasome proteins and proteins of the ERAD system in the cytotoxicity of type II ribosome-inactivating proteins ricin and viscumin was investigated. For this, the cell line of colorectal adenocarcinoma HT29, as well as the HT29-sh002 line obtained on its basis, were used. On the basis on the proteome analysis of these lines and the estimation of the proportion of inactivated ribosomes, it was shown that the contribution of the proteasome to the degradation of the catalytic subunits of toxins is different. The role of the Cdc37 co-chaperone in maintaining the stability of A subunit of viscumin in the cytoplasm is shown.",

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AU - Tikhonova, O. V.

AU - Knyazev, E. N.

AU - Tonevitsky, E. A.

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Relationship between the Expression Level of PSMD11 and Other Proteasome Proteins with the Activity of Ricin and Viscumin

Abstract

Bibliographical note

Funding

Keywords

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