Regulation of the retinoblastoma protein-related protein p107 by G1 cyclin-associated kinases

Z. X Xiao, D Ginsberg, M Ewen, D. M Livingston

Research output: Contribution to journalArticlepeer-review

Abstract

p107 is a retinoblastoma protein-related phosphoprotein that, when overproduced, displays a growth inhibitory function. It interacts with and modulates the activity of the transcription factor, E2F-4. In addition, p107 physically associates with cyclin E-CDK2 and cyclin A-CDK2 complexes in late G1 and at G1/S, respectively, an indication that cyclin-dependent kinase complexes may regulate, contribute to, and/or benefit from p107 function during the cell cycle. Our results show that p107 phosphorylation begins in mid G1 and proceeds through late G1 and S and that cyclin D-associated kinase(s) contributes to this process. In addition, E2F-4 binds selectively to hypophosphorylated p107, and G1 cyclin-dependent p107 phosphorylation leads to the dissociation of p107-E2F-4 complexes as well as inactivation of p107 G1 blocking function.
Original languageAmerican English
Pages (from-to)4633-4637
JournalProceedings of the National Academy of Sciences
Volume93
Issue number10
StatePublished - 1996

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