TY - JOUR
T1 - Regulation of interleukin-2 and interferon-γ gene expression in renal failure
AU - Gerez, Lisya
AU - Madar, Liora
AU - Shkolnik, Tamar
AU - Kristal, Batya
AU - Arad, Gila
AU - Reshef, Ayelet
AU - Steinberger, Asnat
AU - Ketzinel, Mali
AU - Sayar, Dror
AU - Shasha, Shaul
AU - Kaempfer, Raymond
N1 - Funding Information:
Supported in part by grants from the Israel Academy of Sciences and
PY - 1991/8
Y1 - 1991/8
N2 - Regulated expression of interleukin-2 (IL-2) and interferon-γ (IFN-γ) genes, induced in cultured peripheral blood mononuclear cells from patients with end-stage renal disease on hemodialysis (HD; N = 13) or peritoneal dialysis (PD; N = 13), was compared to that of 32 normal donors. Culture conditions were chosen that measure the transient, phytohemagglutinin-induced expression of IL-2 and IFN-γ messenger RNA (mRNA), as well the intactness of post-transcriptional and suppressor T cell-dependent mechanisms that control this expression. The latter was achieved by analyzing the superinduction of IL-2 and IFN-γ mRNA occurring upon culture with cycloheximide or after low-dose γ-irradiation, respectively. HD subjects showed a complete loss of inducibility of the IL-2 gene, concomitant with decreased inducibility of IFN-γ mRNA. In PD subjects, by contrast, expression of IL-2 mRNA was as vigorous as in normal donors, while IFN-γ mRNA was even more strongly inducible. This difference in gene inducibility is caused by a lack of T cell function in HD subjects. The defect in IL-2 gene expression in HD subjects, occurring most likely at transcription, may underly their impaired immune function.
AB - Regulated expression of interleukin-2 (IL-2) and interferon-γ (IFN-γ) genes, induced in cultured peripheral blood mononuclear cells from patients with end-stage renal disease on hemodialysis (HD; N = 13) or peritoneal dialysis (PD; N = 13), was compared to that of 32 normal donors. Culture conditions were chosen that measure the transient, phytohemagglutinin-induced expression of IL-2 and IFN-γ messenger RNA (mRNA), as well the intactness of post-transcriptional and suppressor T cell-dependent mechanisms that control this expression. The latter was achieved by analyzing the superinduction of IL-2 and IFN-γ mRNA occurring upon culture with cycloheximide or after low-dose γ-irradiation, respectively. HD subjects showed a complete loss of inducibility of the IL-2 gene, concomitant with decreased inducibility of IFN-γ mRNA. In PD subjects, by contrast, expression of IL-2 mRNA was as vigorous as in normal donors, while IFN-γ mRNA was even more strongly inducible. This difference in gene inducibility is caused by a lack of T cell function in HD subjects. The defect in IL-2 gene expression in HD subjects, occurring most likely at transcription, may underly their impaired immune function.
UR - http://www.scopus.com/inward/record.url?scp=0025902321&partnerID=8YFLogxK
U2 - 10.1038/ki.1991.209
DO - 10.1038/ki.1991.209
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 1942774
AN - SCOPUS:0025902321
SN - 0085-2538
VL - 40
SP - 266
EP - 272
JO - Kidney International
JF - Kidney International
IS - 2
ER -