Regulation of GDNF expression in cultured astrocytes by inflammatory stimuli

Elena Appel, Ofer Kolman, Gila Kazimirsky, Peter M. Blumberg, Chaya Brodie

    Research output: Contribution to journalArticlepeer-review

    115 Scopus citations


    ASTROCYTES express increased levels of neurotrophic factors in response to pathological conditions in the CNS such as injury and inflammation. We have examined the effects of lipopolysaccharide (LPS) and inflammatory cytokines on the expression of GDNF by mouse astrocytes and by C6 glial cells. LPS and tumor necrosis factor-α (TNF-α) induced an increase in level of glial-derived neurotrophic factor (GDNF) mRNA in both cell types. Similarly, the synthesis of GDNF protein was increased by both treatments. Interleukin-1β (IL-1β) and interferon-γ, (IFN-γ) induced similar effects on GDNF production, whereas IL-2 and IL-6 had no significant effects. These results indicate that the expression of GDNF in astrocytes is regulated by inflammatory stimuli and therefore may provide neurotrophic support to injured neurons in inflammatory conditions in the CNS.

    Original languageEnglish
    Pages (from-to)3309-3312
    Number of pages4
    Issue number15
    StatePublished - 20 Oct 1997


    • Astrocytes
    • Brain inflammation
    • GDNF
    • IL-6
    • LPS
    • TNF-α


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