Recording and hodgkin-huxley kinetic analysis of voltage-gated ion channels in nucleated patches

Mara Almog; Alon Korngreen

doi:10.1007/978-1-4939-3411-9_14

Recording and hodgkin-huxley kinetic analysis of voltage-gated ion channels in nucleated patches

Mara Almog, Alon Korngreen

The Mina and Everard Goodman Faculty of Life Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

The patch-clamp technique is widely used to measure and characterize ion currents flowing through the cell membrane. Various configurations can be used according to the research hypothesis. A relatively new configuration of the patch-clamp technique is the outside-out nucleated patch, which allows measuring somatic currents in voltage-clamp recordings due to a reduction in technical issues. Characterization of ionic currents is mostly based on the Hodgkin-Huxley model, a detailed mathematical and biophysical model of membrane excitability. This chapter describes voltage-clamp experiments step by step, from preparation and performing outside-out nucleated patch experiments through to the Hodgkin-Huxley analysis of the recorded data.

Original language	English
Title of host publication	Neuromethods
Publisher	Humana Press Inc.
Pages	305-323
Number of pages	19
DOIs	https://doi.org/10.1007/978-1-4939-3411-9_14
State	Published - 1 Jan 2016

Publication series

Name	Neuromethods
Volume	113
ISSN (Print)	0893-2336
ISSN (Electronic)	1940-6045

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2016.

Keywords

Hodgkin-Huxley model
Kinetic analysis
Nucleated patch
Outside-out patch
Patch clamp
Voltage-gated channels

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/978-1-4939-3411-9_14

Cite this

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Recording and hodgkin-huxley kinetic analysis of voltage-gated ion channels in nucleated patches

Abstract

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Bibliographical note

Keywords

UN SDGs

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