The patch-clamp technique is widely used to measure and characterize ion currents flowing through the cell membrane. Various configurations can be used according to the research hypothesis. A relatively new configuration of the patch-clamp technique is the outside-out nucleated patch, which allows measuring somatic currents in voltage-clamp recordings due to a reduction in technical issues. Characterization of ionic currents is mostly based on the Hodgkin-Huxley model, a detailed mathematical and biophysical model of membrane excitability. This chapter describes voltage-clamp experiments step by step, from preparation and performing outside-out nucleated patch experiments through to the Hodgkin-Huxley analysis of the recorded data.
|Title of host publication||Neuromethods|
|Publisher||Humana Press Inc.|
|Number of pages||19|
|State||Published - 1 Jan 2016|
Bibliographical notePublisher Copyright:
© Springer Science+Business Media New York 2016.
- Hodgkin-Huxley model
- Kinetic analysis
- Nucleated patch
- Outside-out patch
- Patch clamp
- Voltage-gated channels