Abstract
This paper presents a structured illumination microscopy (SIM) reconstruction algorithm that allows the reconstruction of super-resolved images with 2N + 1 raw intensity images, with N being the number of structured illumination directions used. The intensity images are recorded after using a 2D grating for the projection fringe and a spatial light modulator to select two orthogonal fringe orientations and perform phase shifting. Super-resolution images can be reconstructed from the five intensity images, enhancing the imaging speed and reducing the photobleaching by 17%, compared to conventional two-direction and three-step phase-shifting SIM. We believe the proposed technique will be further developed and widely applied in many fields.
| Original language | English |
|---|---|
| Pages (from-to) | 765-773 |
| Number of pages | 9 |
| Journal | Journal of the Optical Society of America A: Optics and Image Science, and Vision |
| Volume | 40 |
| Issue number | 4 |
| DOIs | |
| State | Published - 1 Apr 2023 |
Bibliographical note
Publisher Copyright:© 2023 Optica Publishing Group.
Funding
Funding. National Natural Science Foundation of China (62075177); National Key Research and Development Program of China (2021YFF0700300, 2022YFE0100700); Ministry of Science, Technology and Space (3-18137); International Cooperation and Exchange Programme (2021-2022); China Scholarship Council; Fundamental Research Funds for the Central Universities (QTZX22039, XJS210504); Key Laboratory of Wuliangye-flavor Liquor Solid-state Fermentation, China National Light Industry (2019JJ012); State Key Laboratory of Transient Optics and Photonics (SKLTOP202001).
| Funders | Funder number |
|---|---|
| Key Laboratory of Wuliangye-flavor Liquor Solid-state Fermentation, China National Light Industry | 2019JJ012 |
| Ministry of Science, Technology and Space | 3-18137 |
| National Natural Science Foundation of China | 62075177 |
| China Scholarship Council | |
| International Cooperation and Exchange Programme | 2021-2022 |
| State Key Laboratory of Transient Optics and Photonics | SKLTOP202001 |
| National Key Research and Development Program of China | 2021YFF0700300, 2022YFE0100700 |
| Fundamental Research Funds for the Central Universities | XJS210504, QTZX22039 |