TY - JOUR
T1 - Real-time optical monitoring of tissue vitality in vivo
AU - Mayevsky, Avraham
AU - Manor, Tamar
AU - Pevzner, Eliyahu
AU - Deutsch, Assaf
AU - Etziony, Revital
AU - Dekel, Nava
PY - 2002
Y1 - 2002
N2 - Evaluation of tissue O2 balance (Supply/Demand) could be done by monitoring in real-time 2 out of the 3 components of the tissue O2 balance equation. In our previous publication (Mayevsky et al, SPIE Vol. 4255:33-39, 2001) we had shown the use of the multiparametric monitoring approach in the neurosurgical operating room, using a device combined of laser Doppler flowmeter (LDF) and surface fluorometer reflectometer. The two instruments having two different light sources, were connected to the tissue via a combined bundle of optical fibers. In order to improve the correlation between tissue blood flow and mitochondrial NADH redox state, the new "Tissue Spectroscope" (TiSpec) that was designed has a single light source and a single bundle of optical fibers: Preliminary results show very clear correlation between TBF and NADH redox state. In addition, the reflected light at the excitation wavelength could be used as an indication for blood volume changes. The results obtained by the TiSpec enabled us to compare tissue O2 delivery (TBF) with O2 balance (NADH redox state) in the brain of gerbils and rats exposed toischemia, anoxia and spreading depression. Real-time monitoring of the metabolic state of the tissue has immense potential during surgical procedures.
AB - Evaluation of tissue O2 balance (Supply/Demand) could be done by monitoring in real-time 2 out of the 3 components of the tissue O2 balance equation. In our previous publication (Mayevsky et al, SPIE Vol. 4255:33-39, 2001) we had shown the use of the multiparametric monitoring approach in the neurosurgical operating room, using a device combined of laser Doppler flowmeter (LDF) and surface fluorometer reflectometer. The two instruments having two different light sources, were connected to the tissue via a combined bundle of optical fibers. In order to improve the correlation between tissue blood flow and mitochondrial NADH redox state, the new "Tissue Spectroscope" (TiSpec) that was designed has a single light source and a single bundle of optical fibers: Preliminary results show very clear correlation between TBF and NADH redox state. In addition, the reflected light at the excitation wavelength could be used as an indication for blood volume changes. The results obtained by the TiSpec enabled us to compare tissue O2 delivery (TBF) with O2 balance (NADH redox state) in the brain of gerbils and rats exposed toischemia, anoxia and spreading depression. Real-time monitoring of the metabolic state of the tissue has immense potential during surgical procedures.
KW - Fiber optics
KW - Laser Doppler flowmetry
KW - NADH redox state
KW - Real-time monitoring
KW - Tissue vitality
UR - http://www.scopus.com/inward/record.url?scp=0036402876&partnerID=8YFLogxK
U2 - 10.1117/12.463821
DO - 10.1117/12.463821
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:0036402876
SN - 0277-786X
VL - 4616
SP - 30
EP - 39
JO - Proceedings of SPIE - The International Society for Optical Engineering
JF - Proceedings of SPIE - The International Society for Optical Engineering
ER -