TY - JOUR
T1 - REACTIONS OF POLYGLUTARALDEHYDE WITH PROTEINS.
AU - Rembaum, A.
AU - Levy, J.
AU - Gupta, A.
AU - Margel, S.
PY - 1978
Y1 - 1978
N2 - Glutaraldehyde is used extensively for the tanning of leather, fixation of living cells, immobilization of enzymes, etc. Its reaction mechanism with proteins is difficult to ascertain since glutaraldehyde in water is in equilibrium with several cyclic and non-cyclic species. This paper presents results of an experimental study which was carried out to synthesize and characterize a reactive glutaraldehyde polymer, and to compare its reactivity with that of a relatively pure monomer. In order to establish reaction conditions which would yield a polymer with a high aldehyde content for further reactions with proteins, the kinetics of polymerization were investigated in aqueous media. The structure of the polymer was elucidated spectrophotometrically, and its reactivity with proteins was compared with that of glutaraldehyde. Human immunoglobulin (IgG) was used as proteins. The results obtained prove that beads derivatized with polyglutaraldehyde bind human IgG more efficiently than those derivatized with glutaraldehyde. The presence of biologically active human IgG on the polyglutaraldehyde beads was ascertained by means of their interaction with human lymphocytes. Therefore the present study led to an improved method of labeling cell subpopulations using immunological techniques.
AB - Glutaraldehyde is used extensively for the tanning of leather, fixation of living cells, immobilization of enzymes, etc. Its reaction mechanism with proteins is difficult to ascertain since glutaraldehyde in water is in equilibrium with several cyclic and non-cyclic species. This paper presents results of an experimental study which was carried out to synthesize and characterize a reactive glutaraldehyde polymer, and to compare its reactivity with that of a relatively pure monomer. In order to establish reaction conditions which would yield a polymer with a high aldehyde content for further reactions with proteins, the kinetics of polymerization were investigated in aqueous media. The structure of the polymer was elucidated spectrophotometrically, and its reactivity with proteins was compared with that of glutaraldehyde. Human immunoglobulin (IgG) was used as proteins. The results obtained prove that beads derivatized with polyglutaraldehyde bind human IgG more efficiently than those derivatized with glutaraldehyde. The presence of biologically active human IgG on the polyglutaraldehyde beads was ascertained by means of their interaction with human lymphocytes. Therefore the present study led to an improved method of labeling cell subpopulations using immunological techniques.
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AN - SCOPUS:0017949031
SN - 0032-3934
VL - 19
SP - 648
EP - 653
JO - American Chemical Society, Polymer Preprints, Division of Polymer Chemistry
JF - American Chemical Society, Polymer Preprints, Division of Polymer Chemistry
IS - 1
T2 - Pap presented at ACS Annu Meet, 175th
Y2 - 12 March 1978 through 17 March 1978
ER -