Abstract
The linoleate oxidation products of the affinity chromatography-purified lipoxygenase-like enzyme isolated from rat testes microsomes were characterized. Three types of reaction products separated by thin-layer chromatography were generally present: polar byproducts (A and B) and hydroperoxides. The methyl hydroxystearates obtained from the enzymically produced hydroperoxides were analysed by gas-liquid chromatography and showed a ratio of 67% 13-hydroxy isomer to 33% 9-hydroxy isomer. The major polar byproduct was analysed by infrared spectra, nuclear magnetic resonance and mass spectrometry (of the toluene-p-sulphonyl derivative) and its structure was established as 13-hydroxy-12-oxo-octadec-cis-9-enoic acid. The possibility of the existence of a linoleate hydroperoxide isomerase in the affinity-purified preparation is discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 293-297 |
| Number of pages | 5 |
| Journal | Biochimica et Biophysica Acta - Lipids and Lipid Metabolism |
| Volume | 572 |
| Issue number | 2 |
| DOIs | |
| State | Published - 26 Feb 1979 |
Keywords
- (Rat testis)
- Hydroperoxide
- Linoleic acid
- Lipoxygenase-like enzyme
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