Rapid microscopy measurement of very large spectral images

Moshe Lindner, Zav Shotan, Yuval Garini

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


The spectral content of a sample provides important information that cannot be detected by the human eye or by using an ordinary RGB camera. The spectrum is typically a fingerprint of the chemical compound, its environmental conditions, phase and geometry. Thus measuring the spectrum at each point of a sample is important for a large range of applications from art preservation through forensics to pathological analysis of a tissue section. To date, however, there is no system that can measure the spectral image of a large sample in a reasonable time. Here we present a novel method for scanning very large spectral images of microscopy samples even if they cannot be viewed in a single field of view of the camera. The system is based on capturing information while the sample is being scanned continuously 'on the fly'. Spectral separation implements Fourier spectroscopy by using an interferometer mounted along the optical axis. High spectral resolution of ∼5 nm at 500 nm could be achieved with a diffraction-limited spatial resolution. The acquisition time is fairly high and takes 6-8 minutes for a sample size of 10mm x 10mm measured under a bright-field microscope using a 20X magnification.

Original languageEnglish
Pages (from-to)9511-9527
Number of pages17
JournalOptics Express
Issue number9
StatePublished - 2 May 2016

Bibliographical note

Funding Information:
This work was supported in part by Applied Spectral Imaging, Yokneam, Israel, the Israel Centers of Research Excellence (ICORE) grant 1902/12 and the Israel Science Foundation grant 51/12.

Publisher Copyright:
© 2016 Optical Society of America.


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