Quantitative Phase Contrast Microscopy with Optimized Partially Coherent Illumination

Kequn Zhuo; Yang Wang; Ying Ma; Sha An; Zeev Zalevsky; Juanjuan Zheng; Peng Gao

doi:10.3390/photonics10040391

Quantitative Phase Contrast Microscopy with Optimized Partially Coherent Illumination

Kequn Zhuo, Yang Wang, Ying Ma, Sha An, Zeev Zalevsky, Juanjuan Zheng, Peng Gao

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

This paper presents a partially coherent illumination quantitative phase contrast microscopic (PCI-QPCM) prototype. In the PCI-QPCM prototype, the light scattered by a rotating diffuser is coupled into a multi-mode fiber, and the output light is used as the illumination for PCI-QPCM. The illumination wave has a constrained spectrum with a diameter of tens of micrometers, which can reduce speckle noise and will not broaden the dc term of the object wave. In the Fourier plane of the object wave, grating-masked phase shifters generated by a spatial light modulator (SLM) allow for measuring the intensity of the undiffracted and diffracted components of the object wave, as well as the phase-shifted interference patterns of the two. Quantitative phase images can be reconstructed from the recorded intensity images. The proposed PCI-QPCM was demonstrated with quantitative phase imaging of a transparent waveguide and a phase-step sample.

Original language	English
Article number	391
Journal	Photonics
Volume	10
Issue number	4
DOIs	https://doi.org/10.3390/photonics10040391
State	Published - Apr 2023

Bibliographical note

Publisher Copyright:
© 2023 by the authors.

Funding

This research was funded by National Natural Science Foundation of China, grant number 62075177; National Key Research and Development Program of China, grant number 2021YFF0700300, 2022YFE0100700; the Natural Science Foundation of Shaanxi Province, grant number 2023JCQN0731, 2023JCYB518; Key Laboratory of Wuliangye-flavor Liquor Solid-state Fermantation, China National Light Industry, grant number 2019JJ012, and the Open Research Fund of State Key Laboratory of Transient Optics and Photonics.

Funders	Funder number
National Natural Science Foundation of China	62075177
Natural Science Foundation of Shaanxi Province	2023JCQN0731, 2019JJ012, 2023JCYB518
State Key Laboratory of Transient Optics and Photonics
National Key Research and Development Program of China	2021YFF0700300, 2022YFE0100700

Keywords

partially coherent illumination
quantitative phase contrast microscopy (QPCM)
spatial light modulator (SLM)

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10.3390/photonics10040391

Cite this

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title = "Quantitative Phase Contrast Microscopy with Optimized Partially Coherent Illumination",

abstract = "This paper presents a partially coherent illumination quantitative phase contrast microscopic (PCI-QPCM) prototype. In the PCI-QPCM prototype, the light scattered by a rotating diffuser is coupled into a multi-mode fiber, and the output light is used as the illumination for PCI-QPCM. The illumination wave has a constrained spectrum with a diameter of tens of micrometers, which can reduce speckle noise and will not broaden the dc term of the object wave. In the Fourier plane of the object wave, grating-masked phase shifters generated by a spatial light modulator (SLM) allow for measuring the intensity of the undiffracted and diffracted components of the object wave, as well as the phase-shifted interference patterns of the two. Quantitative phase images can be reconstructed from the recorded intensity images. The proposed PCI-QPCM was demonstrated with quantitative phase imaging of a transparent waveguide and a phase-step sample.",

keywords = "partially coherent illumination, quantitative phase contrast microscopy (QPCM), spatial light modulator (SLM)",

author = "Kequn Zhuo and Yang Wang and Ying Ma and Sha An and Zeev Zalevsky and Juanjuan Zheng and Peng Gao",

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T1 - Quantitative Phase Contrast Microscopy with Optimized Partially Coherent Illumination

AU - Zhuo, Kequn

AU - Wang, Yang

AU - Ma, Ying

AU - An, Sha

AU - Zalevsky, Zeev

AU - Zheng, Juanjuan

AU - Gao, Peng

PY - 2023/4

Y1 - 2023/4

N2 - This paper presents a partially coherent illumination quantitative phase contrast microscopic (PCI-QPCM) prototype. In the PCI-QPCM prototype, the light scattered by a rotating diffuser is coupled into a multi-mode fiber, and the output light is used as the illumination for PCI-QPCM. The illumination wave has a constrained spectrum with a diameter of tens of micrometers, which can reduce speckle noise and will not broaden the dc term of the object wave. In the Fourier plane of the object wave, grating-masked phase shifters generated by a spatial light modulator (SLM) allow for measuring the intensity of the undiffracted and diffracted components of the object wave, as well as the phase-shifted interference patterns of the two. Quantitative phase images can be reconstructed from the recorded intensity images. The proposed PCI-QPCM was demonstrated with quantitative phase imaging of a transparent waveguide and a phase-step sample.

AB - This paper presents a partially coherent illumination quantitative phase contrast microscopic (PCI-QPCM) prototype. In the PCI-QPCM prototype, the light scattered by a rotating diffuser is coupled into a multi-mode fiber, and the output light is used as the illumination for PCI-QPCM. The illumination wave has a constrained spectrum with a diameter of tens of micrometers, which can reduce speckle noise and will not broaden the dc term of the object wave. In the Fourier plane of the object wave, grating-masked phase shifters generated by a spatial light modulator (SLM) allow for measuring the intensity of the undiffracted and diffracted components of the object wave, as well as the phase-shifted interference patterns of the two. Quantitative phase images can be reconstructed from the recorded intensity images. The proposed PCI-QPCM was demonstrated with quantitative phase imaging of a transparent waveguide and a phase-step sample.

KW - partially coherent illumination

KW - quantitative phase contrast microscopy (QPCM)

KW - spatial light modulator (SLM)

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ER -

Quantitative Phase Contrast Microscopy with Optimized Partially Coherent Illumination

Abstract

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Keywords

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