TY - JOUR
T1 - Quantitative Phase Contrast Microscopy with Optimized Partially Coherent Illumination
AU - Zhuo, Kequn
AU - Wang, Yang
AU - Ma, Ying
AU - An, Sha
AU - Zalevsky, Zeev
AU - Zheng, Juanjuan
AU - Gao, Peng
N1 - Publisher Copyright:
© 2023 by the authors.
PY - 2023/4
Y1 - 2023/4
N2 - This paper presents a partially coherent illumination quantitative phase contrast microscopic (PCI-QPCM) prototype. In the PCI-QPCM prototype, the light scattered by a rotating diffuser is coupled into a multi-mode fiber, and the output light is used as the illumination for PCI-QPCM. The illumination wave has a constrained spectrum with a diameter of tens of micrometers, which can reduce speckle noise and will not broaden the dc term of the object wave. In the Fourier plane of the object wave, grating-masked phase shifters generated by a spatial light modulator (SLM) allow for measuring the intensity of the undiffracted and diffracted components of the object wave, as well as the phase-shifted interference patterns of the two. Quantitative phase images can be reconstructed from the recorded intensity images. The proposed PCI-QPCM was demonstrated with quantitative phase imaging of a transparent waveguide and a phase-step sample.
AB - This paper presents a partially coherent illumination quantitative phase contrast microscopic (PCI-QPCM) prototype. In the PCI-QPCM prototype, the light scattered by a rotating diffuser is coupled into a multi-mode fiber, and the output light is used as the illumination for PCI-QPCM. The illumination wave has a constrained spectrum with a diameter of tens of micrometers, which can reduce speckle noise and will not broaden the dc term of the object wave. In the Fourier plane of the object wave, grating-masked phase shifters generated by a spatial light modulator (SLM) allow for measuring the intensity of the undiffracted and diffracted components of the object wave, as well as the phase-shifted interference patterns of the two. Quantitative phase images can be reconstructed from the recorded intensity images. The proposed PCI-QPCM was demonstrated with quantitative phase imaging of a transparent waveguide and a phase-step sample.
KW - partially coherent illumination
KW - quantitative phase contrast microscopy (QPCM)
KW - spatial light modulator (SLM)
UR - http://www.scopus.com/inward/record.url?scp=85153765544&partnerID=8YFLogxK
U2 - 10.3390/photonics10040391
DO - 10.3390/photonics10040391
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AN - SCOPUS:85153765544
SN - 2304-6732
VL - 10
JO - Photonics
JF - Photonics
IS - 4
M1 - 391
ER -