Light chain amyloidosis (AL) is a bone marrow (BM) plasma cell neoplasia with systemic deposition of Ig light chain amyloid fibrils. Here, we report the identification of clonal CD19 B cells in the BM and the use of a novel mathematical algorithm to generate B cell lineage trees of the clonal CD19 B cells and CD138 plasma cells from the BM of AL patients to delineate the relationship between these two clonal populations. The CD19+ clonal B cells in the BM of AL patients related to the clonal plasma cells represent a pre-plasma cell precursor population. The B cell lineage trees from AL patients also show significant differences in clonal diversification and antigenic selection compared to clones from normal, healthy controls. These data provide a robust example of the use of graphical quantification methods in delineating the role of neoplastic precursors in the pathogenesis of hematopoietic malignancies. © 2006 Elsevier Inc. All rights reserved.
Bibliographical noteFunding Information:
The authors would like to acknowledge the following sources of financial support for this study, the Hematological Malignancies Research Fund, Mayo Foundation (RSA); The Israel Science Foundation (grant number 759/01-1), the Israel Cancer Research Fund, the Human Frontiers Science Program, and the Swedish Foundation for Strategic Research (RM). The authors would also acknowledge the Mayo Dysproteinemia Cell Bank for providing BM and blood samples for the AL and MM patients. The authors thank Ms. Lavonne Knutson for the assistance with manuscript preparation.
- B cells
- Immunoglobulin light chain amyloidosis
- Monoclonal gammopathy
- Plasma cell dyscrasia