@inbook{0b41044ae9014b3db8e96bc3c27854b3,
title = "Quantifying the ratio of spliceosome components assembled on pre-mRNA",
abstract = "RNA processing by the splicing machinery removes intronic sequences from pre-mRNA to generate mature mRNA transcripts. Many splicing events occur co-transcriptionally when the pre-mRNA is still associated with the transcription machinery. This mechanism raises questions regarding the number of spliceosomes associated with the pre-mRNA at a given time. In this protocol, we present a quantitative FISH approach that measures the ratio of intensities between two different spliceosomal components associated on a nascent mRNA, and compares to the number of introns in the mRNA, thereby calculating the number of spliceosome complexes assembled with each transcript.",
keywords = "Immunofluorescence, Intron, RNA FISH, Spliceosome",
author = "Noa Neufeld and Yehuda Brody and Yaron Shav-Tal",
year = "2014",
doi = "10.1007/978-1-62703-980-2_19",
language = "אנגלית",
isbn = "9781627039796",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "257--269",
booktitle = "Spliceosomal Pre-mRNA Splicing",
}