Pyridoxal-thiosemicarbazide: Its anion sensing ability and application in living cells imaging

Darshna Sharma; Anuradha Moirangthem; Rajender Kumar; S. K. Ashok Kumar; Anil Kuwar; John F. Callan; Anupam Basu; Suban K. Sahoo

doi:10.1039/c5ra05059f

Pyridoxal-thiosemicarbazide: Its anion sensing ability and application in living cells imaging

Darshna Sharma, Anuradha Moirangthem, Rajender Kumar, S. K. Ashok Kumar, Anil Kuwar, John F. Callan, Anupam Basu, Suban K. Sahoo

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27 Scopus citations

Abstract

A new anion selective chemosensor L was derived through a direct condensation reaction between pyridoxal and thiosemicarbazide. Sensor L showed selective recognition and sensing ability towards F^- and AcO^- anions through a naked-eye detectable color change from colorless to light yellow, appearance of a new charge transfer absorption band at 404 nm and significant "turn-on" fluorescence at 506 nm. The detection limit of L as a fluorescent 'turn-on' sensor for the analysis of F^- and AcO^- was estimated to be 0.10 μM. The anion sensing mechanisms of L was supported by ¹H NMR and DFT results. Finally, the cytotoxicity effect of L and its ability to image intracellular F^- ions in the living HeLa cells was investigated.

Original language	English
Pages (from-to)	50741-50746
Number of pages	6
Journal	RSC Advances
Volume	5
Issue number	63
DOIs	https://doi.org/10.1039/c5ra05059f
State	Published - 2015
Externally published	Yes

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© The Royal Society of Chemistry 2015.

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Kyungpook National University

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10.1039/c5ra05059f

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abstract = "A new anion selective chemosensor L was derived through a direct condensation reaction between pyridoxal and thiosemicarbazide. Sensor L showed selective recognition and sensing ability towards F- and AcO- anions through a naked-eye detectable color change from colorless to light yellow, appearance of a new charge transfer absorption band at 404 nm and significant {"}turn-on{"} fluorescence at 506 nm. The detection limit of L as a fluorescent 'turn-on' sensor for the analysis of F- and AcO- was estimated to be 0.10 μM. The anion sensing mechanisms of L was supported by 1H NMR and DFT results. Finally, the cytotoxicity effect of L and its ability to image intracellular F- ions in the living HeLa cells was investigated.",

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AU - Sharma, Darshna

AU - Moirangthem, Anuradha

AU - Kumar, Rajender

AU - Ashok Kumar, S. K.

AU - Kuwar, Anil

AU - Callan, John F.

AU - Basu, Anupam

AU - Sahoo, Suban K.

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Pyridoxal-thiosemicarbazide: Its anion sensing ability and application in living cells imaging

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