TY - JOUR
T1 - Purification and characterization of the gonad lectin of Aplysia depilans
AU - Gilboa-Garber, Nechama
AU - Susswein, Abraham J.
AU - Mizrahi, Lea
AU - Avichezer, Dody
PY - 1985/2/25
Y1 - 1985/2/25
N2 - Extracts of gonads and fertilized eggs of Aplysia depilans contain a D-galacturonic and D-galactose-binding lectin. This lectin reacts strongly with rabbit and human erythrocytes independent of ABO blood groups, weakly with dog, mouse, rat, and chick erythrocytes and not at all or very weakly with sheep erythrocytes. Purification of the gonad lectin was easily achieved, with a high yield, by heating to 70°C, precipitation with ammonium sulfate and affinity chromatography on Sepharose 4B. The purified lectin was found to be a glucoprotein of molecular mass around 55-60 kDa; it stimulates mitogenesis of human peripheral lymphocytes.
AB - Extracts of gonads and fertilized eggs of Aplysia depilans contain a D-galacturonic and D-galactose-binding lectin. This lectin reacts strongly with rabbit and human erythrocytes independent of ABO blood groups, weakly with dog, mouse, rat, and chick erythrocytes and not at all or very weakly with sheep erythrocytes. Purification of the gonad lectin was easily achieved, with a high yield, by heating to 70°C, precipitation with ammonium sulfate and affinity chromatography on Sepharose 4B. The purified lectin was found to be a glucoprotein of molecular mass around 55-60 kDa; it stimulates mitogenesis of human peripheral lymphocytes.
KW - Aplysia
KW - Gonad lectin
KW - Lectin purification
KW - Mitogenic lectin
UR - http://www.scopus.com/inward/record.url?scp=0022431919&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(85)80273-8
DO - 10.1016/0014-5793(85)80273-8
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C2 - 4038665
AN - SCOPUS:0022431919
SN - 0014-5793
VL - 181
SP - 267
EP - 270
JO - FEBS Letters
JF - FEBS Letters
IS - 2
ER -