Protofilament Structure and Supramolecular Polymorphism of Aggregated Mutant Huntingtin Exon 1

Jennifer C. Boatz, Talia Piretra, Alessia Lasorsa, Irina Matlahov, James F. Conway, Patrick C.A. van der Wel

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Huntington's disease is a progressive neurodegenerative disease caused by expansion of the polyglutamine domain in the first exon of huntingtin (HttEx1). The extent of expansion correlates with disease progression and formation of amyloid-like protein deposits within the brain. The latter display polymorphism at the microscopic level, both in cerebral tissue and in vitro. Such polymorphism can dramatically influence cytotoxicity, leading to much interest in the conditions and mechanisms that dictate the formation of polymorphs. We examine conditions that govern HttEx1 polymorphism in vitro, including concentration and the role of the non-polyglutamine flanking domains. Using electron microscopy, we observe polymorphs that differ in width and tendency for higher-order bundling. Strikingly, aggregation yields different polymorphs at low and high concentrations. Narrow filaments dominate at low concentrations that may be more relevant in vivo. We dissect the role of N- and C-terminal flanking domains using protein with the former (httNT or N17) largely removed. The truncated protein is generated by trypsin cleavage of soluble HttEx1 fusion protein, which we analyze in some detail. Dye binding and solid-state NMR studies reveal changes in fibril surface characteristics and flanking domain mobility. Higher-order interactions appear facilitated by the C-terminal tail, while the polyglutamine forms an amyloid core resembling those of other polyglutamine deposits. Fibril-surface-mediated branching, previously attributed to secondary nucleation, is reduced in absence of httNT. A new model for the architecture of the HttEx1 filaments is presented and discussed in context of the assembly mechanism and biological activity.

Original languageEnglish
Pages (from-to)4722-4744
Number of pages23
JournalJournal of Molecular Biology
Volume432
Issue number16
DOIs
StatePublished - 24 Jul 2020
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2020 The Author(s)

Keywords

  • Huntington's disease
  • MAS ssNMR
  • TEM
  • amyloid
  • supramolecular assembly

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