Abstract
Blood-brain barrier disruption (BBBD) using focused ultrasound (FUS) and microbubbles (MBs) is an effective tool for therapeutic delivery to the brain. Here, we present an optimized protocol for quantifying fluorescent molecules extravasation in mice. We describe steps for ultrasound treatment, injection of MBs and fluorescent dyes, brain harvesting, microscopy imaging, and image postprocessing algorithm. Our protocol has proven to successfully conduct a diameter-dependent analysis that measures vascular leakage following FUS-mediated BBBD at a single blood vessel resolution. For complete details on the use and execution of this protocol, please refer to Katz et al.1
Original language | English |
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Article number | 102770 |
Journal | STAR Protocols |
Volume | 5 |
Issue number | 1 |
DOIs | |
State | Published - 15 Mar 2024 |
Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2023 The Author(s)
Funding
This work was supported by funding from Insightec Ltd. the Israel Science Foundation (grant numbers 192/ 22 and 3450/20), the Israel Ministry of Science and Technology (grant number 101716), an ERC StG grant (no. 101041118) (NanoBubbleBrain), and a Zimin Institute grant, and was partially supported by a grant from the Nicholas and Elizabeth Slezak Super Center for Cardiac Research and Biomedical Engineering at Tel Aviv University. L.P. assisted with the in vivo experiments, microscopy, and image processing and wrote this manuscript. S.K. designed and performed the research. R.G. revised the manuscript. T.I. advised and designed the research. All authors have approved the final version of this manuscript. The authors declare no competing interests. This work was supported by funding from Insightec Ltd., the Israel Science Foundation (grant numbers 192/ 22 and 3450/20 ), the Israel Ministry of Science and Technology (grant number 101716 ), an ERC StG grant (no. 101041118 ) (NanoBubbleBrain), and a Zimin Institute grant, and was partially supported by a grant from the Nicholas and Elizabeth Slezak Super Center for Cardiac Research and Biomedical Engineering at Tel Aviv University .
Funders | Funder number |
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Insightec Ltd. | |
Insightec Ltd. the Israel Science Foundation | |
European Commission | 101041118 |
Israel Science Foundation | 192/ 22, 3450/20 |
Tel Aviv University | |
Ministry of science and technology, Israel | 101716 |
Nicholas and Elizabeth Slezak Super Center for Cardiac Research and Biomedical Engineering |
Keywords
- Biotechnology and bioengineering
- Microscopy
- Neuroscience
- Physics