Protocol to assess extravasation of fluorescent molecules in mice after ultrasound-mediated blood-brain barrier opening

Lea Peko, Sharon Katz, Roni Gattegno, Tali Ilovitsh

Research output: Contribution to journalArticlepeer-review

Abstract

Blood-brain barrier disruption (BBBD) using focused ultrasound (FUS) and microbubbles (MBs) is an effective tool for therapeutic delivery to the brain. Here, we present an optimized protocol for quantifying fluorescent molecules extravasation in mice. We describe steps for ultrasound treatment, injection of MBs and fluorescent dyes, brain harvesting, microscopy imaging, and image postprocessing algorithm. Our protocol has proven to successfully conduct a diameter-dependent analysis that measures vascular leakage following FUS-mediated BBBD at a single blood vessel resolution. For complete details on the use and execution of this protocol, please refer to Katz et al.1

Original languageEnglish
Article number102770
JournalSTAR Protocols
Volume5
Issue number1
DOIs
StatePublished - 15 Mar 2024
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2023 The Author(s)

Keywords

  • Biotechnology and bioengineering
  • Microscopy
  • Neuroscience
  • Physics

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